Integrin alpha 7 Antibody (8G2) - Azide and BSA Free

Western Blot: Integrin alpha 7 Antibody (8G2) [H00003679-M01] - ITGA7 monoclonal antibody (M01), clone 8G2 Analysis of ITGA7 expression in Jurkat.

Sandwich ELISA: Integrin alpha 7 Antibody (8G2) [H00003679-M01] - Detection limit for recombinant GST tagged ITGA7 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clone: 8G2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Integrin alpha 7 Antibody (8G2) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ITGA7 (NP_002197, 478 a.a. ~ 577 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SHEVSIAPRSIDLEQPNCAGGHSVCVDLRVCFSYIAVPSSYSPTVALDYVLDADTDRRLRGQVPRVTFLSRNLEEPKHQASGTVWLKHQHDRVCGDAMFQ
• Specificity: ITGA7 - integrin, alpha 7
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA7
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Integrin alpha 7 Antibody (8G2) - Azide and BSA Free

• FLJ25220
• integrin alpha 7 chain
• Integrin alpha 7
• integrin alpha-7
• integrin, alpha 7
• ITGA7

Background

ITGA7 encodes integrin alpha chain 7. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 7 undergoes post-translational cleavage within the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form an integrin that binds to the extracellular matrix protein laminin-1. Alpha 7 beta 1 is the major integrin complex expressed in differentiated muscle cells. Splice variants of alpha 7 that differ in both the extracellular and cytoplasmic domains exist in the mouse; however, to date only a single human transcript type has been isolated: it contains extracellular and cytoplasmic domains corresponding to the mouse X2 and B variants, respectively. A unique extracellular splice variant has been identified in human, although it clearly represents a minor species and its biological significance is unclear.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 7 Antibody (H00003679-M01)(2)

Publications using H00003679-M01

• Hammers DW, Sleeper MM, Forbes SC et al. Disease-modifying effects of orally bioavailable NF-kB inhibitors in dystrophin-deficient muscle. JCI Insight 2016-12-22 [PMID: 28018975]
• Ren B, Yu YP, Tseng GC et al. Analysis of integrin alpha7 mutations in prostate cancer, liver cancer, glioblastoma multiforme, and leiomyosarcoma. J Natl Cancer Inst;99(11):868-80. 2007-06-06 [PMID: 17551147] (WB, Human)

Bioinformatics

• Gene Symbol: ITGA7
• Entrez:
  • 3679(Human)
• OMIM:
  • 600536(Human)
• Uniprot:
  • NP_002197(Human)