Integrin alpha 2/CD49b Antibody (430907)

• Reactivity: Hu
• Applications: WB
• Clone: 430907
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin alpha 2/CD49b Antibody (430907) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha 2/CD49b
  Tyr30-Thr1132
  Accession # P17301
• Specificity: Detects human Integrin alpha 2/CD49b in direct ELISAs and Western blots. In direct ELISAs and Western blots, 50‑100% cross‑reactivity with recombinant mouse (rm) Integrin alpha 2 is observed and no cross-reactivity with recombinant human (rh) Integrin alpha 1, alpha 3, alpha 5, alpha 6, alpha 8, alpha 9, alpha 10, alpha 11, alpha L, alpha M, or alpha V is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: ITGA2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 2/CD49b Antibody (430907)

• alpha-2 subunit
• CD49b antigen
• CD49b
• Integrin alpha 2
• integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
• ITGA2
• VLA 2
• VLA-2 alpha

Background

Integrin alpha 2 is one of twelve integrin family alpha subunits that share the beta 1 subunit (1‑3). Integrin alpha 2 beta 1 is the non-covalent heterodimer of 160 kDa alpha 2 (CD49b) and 130 kDa beta 1 (CD29) type I transmembrane glycoprotein subunits and is one of six very late antigens on activated T cells, designated VLA2 (3). The alpha 2 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 3). The beta 1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1, 2). The 1102 amino acid (aa) human alpha 2 extracellular domain (ECD) shares 83‑89% aa sequence identity with mouse, rat, canine, bovine and equine alpha 2. The I domain-containing beta 1 integrins ( alpha 1 beta 1, alpha 2 beta 1, alpha 10 beta 1 and alpha 11 beta 1) all bind collagens, with alpha 2 beta 1 preferring collagens I‑III (4, 5). Platelet alpha 2 beta 1, also called GPIa, cooperates with another adhesion protein, GPVI, to coordinate platelet collagen binding and activation (3, 6, 7). Other alpha 2 beta 1 ligands include laminin, decorin, E-cadherin, and collagen-like regions of collectin molecules such as C1q (4). Adhesion is synergized by crosstalk with syndecan-1 or HGF R/c-Met, and antagonized by crosstalk with Integrin alpha 1 beta 1 (8‑10). In addition to expression on selected hematopoietic cells, alpha 2 beta 1 is present on a wide variety of non-hematopoietic cells (4). Mice deficient in the alpha 2 subunit have defects in innate immune responses, wound mast cell infiltration and angiogenesis, and platelet responses to collagen (6, 11, 12). In innate immunity, alpha 2 beta 1 binding to C1q initiates the complement cascade and costimulates mast cell activation, triggering neutrophil influx (4, 12).

1. Takada, Y. et al. (2007) Genome Biol. 8:215.
2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
3. Takada, Y. and M.E. Hemler (1989) J. Cell Biol. 109:397.
4. Zutter, M.M. and B.T Edelson (2007) Immunobiology 212:343.
5. McCall-Culbreath, K.D. and M.M. Zutter (2008) Curr. Drug Targets 9:139.
6. Sarratt, K.L. et al. (2005) Blood 106:1268.
7. Lecut, C. et al. (2005) Thromb. Haemost. 94:107.
8. Vuoriluoto, K. et al. (2008) Exp. Cell Res. 314:3369.
9. McCall-Culbreath, K.D. et al. (2008) Blood 111:3562.
10. Abair, T.D. et al. (2008) Exp. Cell Res. 314:3593.
11. Zweers, M. et al. (2006) J. Invest. Dermatol. 127:467.
12. Edelson, B.T. et al. 2006) Blood 107:143.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 2/CD49b Antibody (MAB12331)(3)

Publications using MAB12331

• EJ Hunter, SW Hamaia, PS Kim, JM Malcor, RW Farndale The effects of inhibition and siRNA knockdown of collagen-binding integrins on human umbilical vein endothelial cell migration and tube formation Scientific Reports, 2022-12-14;12(1):21601. 2022-12-14 [PMID: 36517525] (Western Blot, Human)
• LB Gong, C Zhang, RX Yu, C Li, YB Fan, YP Liu, XJ Qu FKBP10 Acts as a New Biomarker for Prognosis and Lymph Node Metastasis of Gastric Cancer by Bioinformatics Analysis and in Vitro Experiments Onco Targets Ther, 2020-07-28;13(0):7399-7409. 2020-07-28 [PMID: 32801763] (Western Blot, Human)
• Hu C, Fang R, Wang K, Luk B, Thamphiwatana S, Dehaini D, Nguyen P, Angsantikul P, Wen C, Kroll A, Carpenter C, Ramesh M, Qu V, Patel S, Zhu J, Shi W, Hofman F, Chen T, Gao W, Zhang K, Chien S, Zhang L Nanoparticle biointerfacing by platelet membrane cloaking. Nature, 2015-09-16;526(7571):118-21. 2015-09-16 [PMID: 26374997] (Western Blot, Human)

Bioinformatics

• Gene Symbol: ITGA2
• Uniprot:
  • P17301()