Integrin alpha 2/CD49b Antibody (235033) [Unconjugated]

Mouse splenocytes were stained with (A) Rat Anti-Mouse Integrin a2/CD49b Monoclonal Antibody (Catalog # MAB1740) or (B) Rat IgG2B isotype control antibody (MAB006), followed by APC-conjugated Anti-Rat IgG Secondary Antibody (F0113) and Rat anti-Mouse NKp46 PE-conjugated Monoclonal Antibody (FAB22252P). Staining was performed using our Staining Membrane-associated Proteins protocol.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 235033
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin alpha 2/CD49b Antibody (235033) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 2/CD49b
  Tyr27-Thr1129
  Accession # Q62469
• Specificity: Detects mouse Integrin alpha 2/CD49b in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Integrin alpha 3, alpha 4, alpha 5, alpha E, or alpha V is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: ITGA2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 2/CD49b Antibody (235033) [Unconjugated]

• alpha-2 subunit
• CD49b antigen
• CD49b
• Integrin alpha 2
• integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
• ITGA2
• VLA 2
• VLA-2 alpha

Background

The Integrin alpha 2 subunit, also known as CD49b and VLA-2 alpha subunit, forms a heterodimer with the Integrin alpha 1 (CD29) or alpha 4 (CD104) subunits and binds Laminin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGA2
• Uniprot:
  • Q62469()