Insulysin/IDE Antibody (334501) [Alexa Fluor® 350]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
334501
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 350
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Insulysin/IDE Antibody (334501) [Alexa Fluor® 350] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Insulysin/IDE
Met42-Leu1019
Accession # P14735
Specificity
Detects human Insulysin/IDE in direct ELISAs and Western blots.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IDE
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Insulysin/IDE Antibody (334501) [Alexa Fluor® 350]

  • Abeta-degrading protease
  • EC 3.4.24
  • EC 3.4.24.56
  • FLJ35968
  • IDE
  • INSDEGM
  • Insulin protease
  • Insulinase
  • insulin-degrading enzyme
  • Insulysin

Background

Insulysin, or insulin-degrading enzyme (IDE), is a zinc metallopeptidase of the inverzincin family. IDE is primarily located in the cytosol, but has been detected as a secreted enzyme and associated with the plasma membrane as well (1). The enzyme is expressed in many tissues, with the highest levels in liver, kidney, brain, and testis (2). IDE hydrolyzes a variety of regulatory peptides, including insulin, glucagon, atrial natriuretic factor, and transforming growth factor-alpha in vitro (1). In addition, IDE has been shown to degrade the amyloid beta (A beta ) peptide, which polymerizes into the plaques associated with Alzheimer's disease (3). Deficiencies in IDE activity may contribute to the pathogenesis of type 2 diabetes mellitus (DM2) and Alzheimer's disease. The IDE region of human chromosome 10q has been genetically linked to DM2 (4). When the IDE gene was specifically disrupted in mice, IDE -/- animals developed hyperinsulinemia and glucose intolerance, characteristics of DM2 (5). The IDE -/- mice were also shown to have a significant decrease in A beta degradation in the brain, resulting in increased cerebral accumulation of A beta peptide. This in vivo evidence is consistent with the hypotheses that IDE is important for the degradation of insulin in cells and for the clearance of A beta peptide in the brain.

  1. Affholter, J. A. et al. (1988) Science 242:1415.
  2. Duckworth, W.C. et al. (1998) Endocr. Rev. 19:608.
  3. Akiyama, H. et al. (1990) Biochem. Biophys. Res. Commun. 170:1325.
  4. Selkoe, D.J. (2001) Neuron 32:177.
  5. Ghosh, S. et al. (2000) Am. J. Hum. Genet. 67:1174.
  6. Farris, W. et al. (2003) Proc. Natl. Acad. Sci. USA 100:4162.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IDE
Uniprot