Inorganic Pyrophosphatase/PPA1 Antibody (4G4) - BSA Free

Western Blot: Inorganic Pyrophosphatase/PPA1 Antibody (4G4) [NBP2-59471] - Lane 1: HeLa cell lysates, Lane 2: A431 cell lysates, Lane 3: 293T cell lysates, Lane 4: HepG2 cell lysates, Lane 5: U87MG cell lysates, Lane 6: PC3 cell lysates, Lane 7: WiDr cell lysates

Immunocytochemistry/Immunofluorescence: Inorganic Pyrophosphatase/PPA1 Antibody (4G4) [NBP2-59471] - Immunofluorescence analysis of Pyrophosphatase/PPA1 in HeLa cells using NBP2-59471 (1:100). The secondary antibody (green) used was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Flow Cytometry: Inorganic Pyrophosphatase/PPA1 Antibody (4G4) [NBP2-59471] - Flow cytometry analysis of Pyrophosphatase/PPA1 in HeLa cells. The cell was stained with NBP2-59471 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa Fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 4G4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Inorganic Pyrophosphatase/PPA1 Antibody (4G4) - BSA Free Summary

• Immunogen: Recombinant human Inorganic Pyrophosphatase/PPA1 (1-289aa) purified from E. coli
• Isotype: IgG2b Lambda
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:10-1:1071
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Inorganic Pyrophosphatase/PPA1 Antibody (4G4) - BSA Free

• diphosphate phosphohydrolase
• EC 3.6.1.1
• inorganic diphosphatase
• inorganic pyrophosphatase 1
• inorganic pyrophosphatase
• IOPPP
• IOPPPMGC111556
• IPP1
• PP1
• PPA1
• PPase
• PPcytosolic inorganic pyrophosphatase
• pyrophosphatase (inorganic) 1
• pyrophosphatase (inorganic)
• pyrophosphatase 1
• Pyrophosphate phospho-hydrolase
• SID6-8061

Background

Pyrophosphatase is encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPA1