ING4 Antibody

Images

 
Western Blot: ING4 Antibody [NB100-1682] - Used at 1.0 ug/ml to detect ING4 (lane 2) present in a U2OS whole cell lysate over expressing the protein. A control lysate (lane 3) shows no background staining. Comparison to ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

ING4 Antibody Summary

Immunogen
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 164-175 of Human p29 ING4 protein (Inhibitor of growth family, member 4). This sequence only shows homology to isoform 1 for ING4.
Specificity
ING4
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ING4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • ELISA 1:3000-1:12000
Application Notes
This affinity-purified antibody has been tested for use in ELISA against the immunizing peptide. Specific conditions for western blotting reactivity should be optimized by the end user. Expect a band at approximately 29 kDa in size corresponding - ING4 isoform 1 by western blotting in the appropriate cell lysate or extract.
Positive Control
ING4 Lysate (NBL1-11992)
Control Peptide
ING4 Protein (NBP1-96942)

Reactivity Notes

Reactivity occurs against human p29 ING4 protein. The sequence is not present in isoforms 2 However, 100% homology is on record for this protein from human, dog, rat, and mouse. Cross reactivity with p29 ING4 protein from chimpanzee and chicken sources is also expected based on high homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ING4 Antibody

  • candidate tumor suppressor p33 ING1 homolog
  • inhibitor of growth family, member 4
  • inhibitor of growth protein 4
  • MGC12557
  • my036
  • p29ING4brain my036 protein

Background

p29 ING4 is a tumor suppressor protein similar to ING1 that may inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. p29 ING4 has been shown to suppress brain tumor angio-genesis through transcriptional repression of RelA/ NFKB3 target genes when complexed with RelA. p29 ING4 may also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. p29 ING4 shows a nuclear localization and interacts with EP300, TP53, RelA, inhibits cell growth, and induces apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. Multiple alternatively spliced transcript variants have been observed. The accession number listed below is for variant (1) that encodes the longest isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ING4
Entrez