Importin alpha 2/KPNA2 Antibody (682239) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 682239
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

Importin alpha 2/KPNA2 Antibody (682239) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human Importin α2/KPNA2
  Ser2-Asp132
  Accession # P52292
• Specificity: Detects human Importin alpha 2/KPNA2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human KPNA1, A3, A4, A5, or B1 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Importin alpha 2/KPNA2 Antibody (682239) [Alexa Fluor® 350]

• Importin alpha 1
• Importin alpha 2
• importin-alpha-P1
• IPOA1
• karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
• Karyopherin subunit alpha-2
• KPNA2
• Pendulin
• QIP2
• QIP2importin alpha 2
• RAG cohort 1
• RAG cohort protein 1
• RCH1
• RCH1importin subunit alpha-2
• SRP1
• SRP1alpha
• SRP1-alpha

Background

Importin alpha 2, also known as KPNA2 (Karyopherin subunit alpha 2), SRP1a, or RAG1, is a 58-60 kDa member of the Importin alpha family of proteins. It is ubiquitously expressed, and found in both nucleus and cytoplasm. Importin alpha 2 functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. For the MRN DNA repair complex, Importin alpha 2 first binds to NBS1, and then to Importin beta 1, which mediates movement through the nuclear pore. Human Importin alpha 2 is 529 amino acids (aa) in length. It contains an N-terminal IBB/Importin beta domain (aa 2-60), ten Armadillo repeats that bind “cargo” (aa 71-496) and two intervening NLS binding sites. There is one splice variant that shows a deletion of aa 72-310, coupled to an 11 aa substitution for aa 389-406. Over aa 1-132, human Importin alpha 2 shares 94% aa identity with mouse Importin alpha 2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.