ILK Antibody

Western Blot: ILK Antibody [NB100-809] - (0.01ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: ILK Antibody [NB100-809] - (4ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Western Blot: ILK Antibody [NB100-809] - (0.03ug/ml) staining of Pig (A), Mouse (B) and Rat (C) Liver lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ILK Antibody Summary

• Description: Novus Biologicals Goat ILK Antibody (NB100-809) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-ILK Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DMIVPILEKMQDK corresponding to C-Terminus according to NP_004508.1, NP_001014794.1, NP_001014795.1.
• Specificity: All 3 varients (represented by NP_004508.1; NP_001014794.1 and NP_001014795.1) encode the same protein.
• Predicted Species: Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ILK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2-4ug/ml
  • Immunohistochemistry-Paraffin 2-4ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.01-0.03ug/ml
• Application Notes: Western blot: Approx. 37kDa band observed in Human, Mouse, Rat and Pig Liver lysate (calculated MW of 34.7kDa according to NP_000036.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.
  IHC: In paraffin embedded Human Kidney shows preferential staining of PCT.
• Control:

  A-431 Whole Cell Lysate
  ILK Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ILK Antibody

• 59 kDa serine/threonine-protein kinase
• DKFZp686F1765
• EC 2.7.11.1
• ESTM24
• ILK
• ILK1
• ILK-1
• ILK2
• ILK-2
• integrin-linked kinase
• integrin-linked kinase-2
• integrin-linked protein kinase
• p59
• p59ILK

Background

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ILK Antibody (NB100-809)(1)

Publication using NB100-809

• Hannigan GE, Leung-Hagesteijn C, Fitz-Gibbon L et al. Regulation of cell adhesion and anchorage-dependent growth by a new beta 1-integrin-linked protein kinase. Nature 1996-01-04 [PMID: 8538749]

Bioinformatics

• Gene Symbol: ILK
• Entrez:
  • 3611(Human)
  • 11846(Mouse)
  • 29221(Rat)
• Uniprot:
  • NP_004508.1(Human)