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ILF2 Antibody

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Western Blot: ILF2 Antibody [NBP1-00204] - Analysis of ILF2 in Human Tonsil lysate (35ug protein in RIPA buffer) using this antibody at 0.03ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: ILF2 Antibody [NBP1-00204] - Analysis of ILF2 in Human Tonsil lysate (35ug protein in RIPA buffer) using this antibody at 0.03ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ILF2 Antibody [NBP1-00204] - (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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ILF2 Antibody Summary

Immunogen
Peptide with sequence C-EKAYEKPPEKKEG corresponding to internal region (near C-Terminus) according to NP_004506.2.
Predicted Species
Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ILF2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 0.03 - 0.1 ug/ml
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:4000
Application Notes
IP: An anonymous customer has reported PAR-CLIP (specific IP procedure) usage is reported in scientific literature (PMID: 20644507). WB: Approx. 43 kDa band observed in human tonsil and thymus lysates (calculated MW of 43.1 kDa band according to NP_004506.2).
IHC-P: Human spleen shows nuclear staining in selected splenocytes.
Control
ILF2 Overexpression Lysate
ILF2 Knockout 293T Cell Lysate
Publications
Read Publication using NBP1-00204.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ILF2 Antibody

  • interleukin enhancer binding factor 2, 45kD
  • interleukin enhancer binding factor 2, 45kDa
  • interleukin enhancer-binding factor 2
  • MGC8391
  • NF45PRO3063
  • Nuclear factor of activated T-cells 45 kDa
  • nuclear factor of activated T-cells, 45-kDa

Background

Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of this gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: ILF3 Antibody (JG37-63) [NBP2-75540] - Analysis of ILF3 on rat heart tissue (1) and 293 cell (2) lysate using anti-ILF3 antibody at 1/1,000 dilution.
Immunocytochemistry/Immunofluorescence: ILF3 Antibody (JG37-63) [NBP2-75540] - Staining ILF3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: ILF3 Antibody (JG37-63) [NBP2-75540] - Analysis of paraffin-embedded mouse kidney tissue using anti-ILF3 antibody. Counter stained with hematoxylin.
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202-IL
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Western Blot: DNA-PKcs Antibody (3H6) [NBP2-22128] - HeLa (A), MCF7 (lB) and HEK293 (C) whole cell protein was separated by SDS-PAGE on 6% gel, transferred to PVDF and probed with NBP2-22128. A single band of DNA PKcs reactivity at approximately 460 kDa was detected in all lysates.
Immunocytochemistry/Immunofluorescence: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis of Hela cells using DNA PKcs mouse mAb (green). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).
Immunohistochemistry-Paraffin: DNA-PKcs Antibody (3H6) [NBP2-22128] - Analysis of paraffin-embedded lung cancer (left) and brain tissues (right) using DNA PKcs mouse mAb with DAB staining.
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Immunohistochemistry-Paraffin: PKR Antibody [NBP1-77266] - Staining of mouse lung tissue with antibody at 5 ug/mL.
Western Blot: PKR Antibody [NBP1-77266] - IAV via Protein Expression Profile in Cell Lines. Antibodies PKR and beta-actin. Secondary: Goat anti-rabbit IgG HRP conjugate.
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Immunocytochemistry/Immunofluorescence: Ku80/XRCC5 Antibody (3D8) [H00007520-M02] - Analysis of monoclonal antibody to XRCC5 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (3D8) [H00007520-M02] - Analysis of monoclonal antibody to XRCC5 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.
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(1 Review)
(4 Publications)
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Western Blot: STRBP Antibody [NBP2-20522] - Sample (30 ug of whole cell lysate) A: IMR32 7. 5% SDS PAGE gel, diluted at 1:1000.
Species: Hu
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Western Blot: RNA Helicase A Antibody [NB110-40579] - Detection of Human DHX9 by Western Blot. Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX9 antibody NB110-40579 used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
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Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Analysis was performed using RbAp48 antibody [13D10]. EasyBlot HRP-conjugated anti mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RbAp48 protein stained by RbAp48 antibody [13D10] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.
Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Detection of RbAp48 in HeLa nuclear extract
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Western Blot: p97/VCP Antibody [NB100-1558] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiVCP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Western Blot: p97/VCP Antibody [NB100-1558] - Detection of human VCP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VCP antibody NB100-1558 used for IP at 3 ug per reaction. VCP was also immunoprecipitated by rabbit anti-VCP antibody NB100-1557. For blotting immunoprecipitated VCP, NB100-1558 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
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Simple Western: KHSRP Antibody [NBP1-18910] - Simple Western lane view shows a specific band for KHSRP in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunohistochemistry: KHSRP Antibody [NBP1-18910] - Immunofluorescence of ILF3 and KHSRP in the retina. Retinal sections were prepared from control mice and from mice at one day after light exposure and stained for GS (green) and KHSRP (red). Image collected and cropped by CiteAb from the following publication (http://bmcbiol.biomedcentral.com/articles/10.1186/s12915-015-0137-1), licensed under a CC-BY licence.
Western Blot: KHSRP Antibody [NBP1-18910] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. KSRP was also immunoprecipitated by rabbit anti-KSRP antibody NBP1-18911.
(1 Review)
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Species: Hu, Mu, Rt
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Western Blot: Exportin-5 Antibody [NBP1-89606] - Analysis using Anti-XPO5 antibody NBP1-89606 (A) shows similar pattern to independent antibody NBP1-89605 (B).
Immunocytochemistry/Immunofluorescence: Exportin-5 Antibody [NBP1-89606] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Exportin-5 Antibody [NBP1-89606] - Staining in human testis and liver tissues using anti-XPO5 antibody. Corresponding XPO5 RNA-seq data are presented for the same tissues.
Species: Hu, Mu, Rt
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Ku70/XRCC6
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Immunocytochemistry/Immunofluorescence: Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells using followed by goat anti-Mouse IgG-CF488 (Green). Phalloidin (Red) is used to label cell membrane.
Flow (Intracellular): Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - An intracellular stain was performed on Hek293 cells with Ku70/XRCC6 Antibody (KU729) NBP2-34663AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image using the Azide and BSA Free format of this antibody.
Flow Cytometry: Ku70/XRCC6 Antibody (KU729) [NBP2-34247] - Flow Cytometry of human Ku (p70/p80) on K562 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Ku70/XRCC6 Antibody (KU729).
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Western Blot: Cyclophilin 40 Antibody [NB120-3562] - Analysis of rat spleen extracts.
Immunocytochemistry/Immunofluorescence: Cyclophilin 40 Antibody [NB120-3562] - Analysis of Cyclophilin D in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cyclophilin D polyclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cyclophilin D staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.
Immunohistochemistry-Paraffin: Cyclophilin 40 Antibody [NB120-3562] - Both normal and cancer biopsies of deparaffinized human Hepatocarcinoma tissue.
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ILF2 Antibody
NBP1-00204
ILF2 Antibody
Species: Hu, Mu, Rt, Ca
Applications: WB, IHC, IHC-P, PEP-ELISA

Publications for ILF2 Antibody (NBP1-00204)(1)


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Control Lysate(s)

ILF2 Overexpression Lysate
ILF2 Knockout 293T Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ILF2
Entrez
Uniprot