ILF2 Antibody

Images

 
Western Blot: ILF2 Antibody [NBP1-00204] - Analysis of ILF2 in Human Tonsil lysate (35ug protein in RIPA buffer) using this antibody at 0.03ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ILF2 Antibody [NBP1-00204] - (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ILF2 Antibody Summary

Immunogen
Peptide with sequence C-EKAYEKPPEKKEG corresponding to internal region (near C-Terminus) according to NP_004506.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ILF2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.03 - 0.1 ug/ml
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:4000
Application Notes
IP: An anonymous customer has reported PAR-CLIP (specific IP procedure) usage is reported in scientific literature (PMID: 20644507). WB: Approx. 43 kDa band observed in human tonsil and thymus lysates (calculated MW of 43.1 kDa band according to NP_004506.2).
IHC-P: Human spleen shows nuclear staining in selected splenocytes.
Control
ILF2 Lysate (NBL1-11972)
ILF2 Lysate (NBP2-65619)
Publications
Read Publication using NBP1-00204.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ILF2 Antibody

  • interleukin enhancer binding factor 2, 45kD
  • interleukin enhancer binding factor 2, 45kDa
  • interleukin enhancer-binding factor 2
  • MGC8391
  • NF45PRO3063
  • Nuclear factor of activated T-cells 45 kDa
  • nuclear factor of activated T-cells, 45-kDa

Background

Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of this gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ILF2 Antibody (NBP1-00204)(1)


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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol ILF2
Entrez
Uniprot