ILF2 Antibody

Western Blot: ILF2 Antibody [NBP1-00204] - Analysis of ILF2 in Human Tonsil lysate (35ug protein in RIPA buffer) using this antibody at 0.03ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: ILF2 Antibody [NBP1-00204] - (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ILF2 Antibody Summary

• Immunogen: Peptide with sequence C-EKAYEKPPEKKEG corresponding to internal region (near C-Terminus) according to NP_004506.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ILF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.03 - 0.1 ug/ml
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:4000
• Application Notes: IP: An anonymous customer has reported PAR-CLIP (specific IP procedure) usage is reported in scientific literature (PMID: 20644507). WB: Approx. 43 kDa band observed in human tonsil and thymus lysates (calculated MW of 43.1 kDa band according to NP_004506.2).
  IHC-P: Human spleen shows nuclear staining in selected splenocytes.
• Positive Control:

  ILF2 Lysate (NBL1-11972)
  ILF2 Lysate (NBP2-65619)

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ILF2 Antibody

• interleukin enhancer binding factor 2, 45kD
• interleukin enhancer binding factor 2, 45kDa
• interleukin enhancer-binding factor 2
• MGC8391
• NF45PRO3063
• Nuclear factor of activated T-cells 45 kDa
• nuclear factor of activated T-cells, 45-kDa

Background

Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of this gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ILF2 Antibody (NBP1-00204)(1)

Publication using NBP1-00204

• Wang J, Rao S, Chu J et al. A protein interaction network for pluripotency of embryonic stem cells. Nature 2006 Nov 16 [PMID: 17093407]

Bioinformatics

• Gene Symbol: ILF2
• Entrez:
  • 3608(Human)
  • 67781(Mouse)
  • 310612(Rat)
• Uniprot:
  • NP_004506.2(Human)