IL-4I1 Antibody (1006231) [Unconjugated]

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM of PMA for 24 hours followed by 10 μg/mL of LPS for 3 hours. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human IL-4I1 Monoclonal Antibody (Catalog # MAB56843) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-4I1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

THP-1 human cell line was stained with Mouse Anti-Human IL-4l1 Monoclonal Antibody (Catalog # MAB56843, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by APC-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: WB, CyTOF-ready, Flow
• Clone: 1006231
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

IL-4I1 Antibody (1006231) [Unconjugated] Summary

• Immunogen: Chinese Hamster Ovary cell line, CHO-derived human IL-4I1
  Met1-His567
  Accession # Q96RQ9
• Specificity: Detects human IL-4I1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-4I1 Antibody (1006231) [Unconjugated]

• Fig-1 protein
• FIG1
• hFIG1
• IL4I1
• IL-4I1
• IL4-induced protein 1
• interleukin 4 induced 1
• interleukin four induced 1
• LAAO
• L-amino-acid oxidase
• LAO
• UNQ636/PRO1265

Background

Interleukin 4 induced protein 1 (IL-4I1), also known as protein FIG-1 and L-amino acid oxidase, is encoded by a B-cell IL-4-inducible gene, FIG1, and is highly expressed in primary metastinal B-cell lymphomas (1-4). It belongs to the flavin monoamine oxidase family, FIG1 subfamily. Enzymological characterization reveals that IL-4I1 has L-amino acid oxidase activity with preference toward aromatic amino acids. Studies have shown that hIL-4I1 inhibited the proliferation of CD3‑stimulated T lymphocytes with a similar effect on CD4(+) and CD8(+) T cells (5). Its inhibitory effect was dependent on enzymatic activity and H₂O₂ production. Its restricted expression to lymphoid tissues indicates that it may play an important function in the immune system (1, 4).

1. Chu, C.C. and W.E. Paul. (1997) Proc. Natl. Acad. Sci. USA 94:2507.
2. Mason, J.M. et al. (2004) J. Immunol. 173:4561.
3. Chavan, S.S. et al. (2002) Biochim. Biophys. Acta. 1576:70.
4. Copie-Bergman, C. et al. (2003) Blood 101:2756.
5. Boulland, M.L. et al. (2007) Blood 110:220.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q96RQ9()