Recombinant Human IL-36 gamma/IL-1F9 Protein

SDS-Page: Recombinant Human IL-36 gamma/IL-1F9 Protein [NBP2-34892]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human IL-36 gamma/IL-1F9 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 169 amino acids corresponding to IL-36 gamma/IL-1F9 Source: E. coli

  Uniprot ID: Q9NZH8

  Amino Acid Sequence: MRGTPGDADG GGRAVYQSMC KPITGTINDL NQQVWTLQGQ NLVAVPRSDS VTPVTVAVIT CKYPEALEQG RGDPIYLGIQ NPEMCLYCEK VGEQPTLQLK EQKIMDLYGQ PEPVKPFLFY RAKTGRTSTL ESVAFPDWFI ASSKRDQPII LTSELGKSYN TAFELNIND

• Details of Functionality: Fully biologically active when compared to standard. The specific activity is determined by its binding ability in a functional ELISA. Immobilized rHuIL-36gamma at 1 ug/mL can bind recombinant human IL-1 Rrp2 Fc Chimera with a range of 0.15-5 ug/mL.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL36G
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of IL-36 gamma/IL-1F9 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 18.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human IL-36 gamma/IL-1F9 Protein

• IL-1 epsilon
• IL-1 H1
• IL-1 Related Protein 2
• IL-1(EPSILON)
• IL1E
• IL-1-epsilon
• IL1F9
• IL-1F9
• IL1H1
• IL-1H1
• IL-1-Related Protein 2
• IL1RP2
• IL-1rp2
• IL36 gamma
• IL-36 gamma
• IL36G
• interleukin 1 family, member 9
• interleukin 1-related protein 2
• Interleukin 36, Gamma
• Interleukin-1 epsilon
• interleukin-1 family member 9
• Interleukin-1 homolog 1
• Interleukin-36 Gamma

Background

Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36alpha, IL-36beta, and IL-36gamma (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. IL-36gamma is secreted when transfected into 293-T cells and it could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1). Furthermore, IL-36gamma also can function as an agonist of NF-kappa B activation through the orphan IL-1- receptor-related protein 2. Recombinant human IL-36gamma is synthesized as a 19 kDa, 169 amino acid (a.a.) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site. Human to mouse, IL-36gamma shares 53 % a.a. identity. Within the family, IL-36gamma shares about 25 % ~ 55 % a.a. sequence identity with IL-1RA, IL-1beta, IL-36RA, IL-36alpha, IL-37, IL-36beta and IL-1F10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for IL-36 gamma/IL-1F9 Recombinant Protein (NBP2-34892). (Showing 1 - 1 of 1 FAQ).

1. Could you let us know the following information?1) Lyophilized buffer formulation (e.g. Lyophilized from PBS.)2) Preservative/Concentration
   • This protein is supplied in the lyophilized format from the same associated lab of ours, and can be used in the functional assays in vivo (so should not have any preservative and endotoxin-tested. Our lab's databases show that E. coli, lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, and no preservative.er.

Bioinformatics

• Gene Symbol: IL36G
• Uniprot:
  • Q9NZH8()
  • Q9NZH8()
  • Q9NZH8()