Recombinant Human IL-36 gamma/IL-1F9 Protein

SDS-Page: Recombinant Human IL-36 gamma/IL-1F9 Protein [NBP2-34891]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human IL-36 gamma/IL-1F9 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 152 amino acids corresponding to IL-36 gamma/IL-1F9 Source: E. coli

  Uniprot ID: Q9NZH8

  Amino Acid Sequence: SMCKPITGTI NDLNQQVWTL QGQNLVAVPR SDSVTPVTVA VITCKYPEAL EQGRGDPIYL GIQNPEMCLY CEKVGEQPTL QLKEQKIMDL YGQPEPVKPF LFYRAKTGRT STLESVAFPD WFIASSKRDQ PIILTSELGK SYNTAFELNI ND

• Details of Functionality: IL-36 gamma/IL-1F9 protein is fully biologically active when compared to standard. The ED50 as determined by its ability to induce IL-8 secretion by human preadipocytes is less than 10 ng/ml, corresponding to a specific activity of > 1 x 10^5 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL36G
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of IL-36 gamma/IL-1F9 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human IL-36 gamma/IL-1F9 Protein

• IL-1 epsilon
• IL-1 H1
• IL-1 Related Protein 2
• IL-1(EPSILON)
• IL1E
• IL-1-epsilon
• IL1F9
• IL-1F9
• IL1H1
• IL-1H1
• IL-1-Related Protein 2
• IL1RP2
• IL-1rp2
• IL36 gamma
• IL-36 gamma
• IL36G
• interleukin 1 family, member 9
• interleukin 1-related protein 2
• Interleukin 36, Gamma
• Interleukin-1 epsilon
• interleukin-1 family member 9
• Interleukin-1 homolog 1
• Interleukin-36 Gamma

Background

Interleukin-36 (IL-36) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL-36alpha, IL-36beta, and IL-36gamma (formerly IL-1F6, IL-1F8, and IL-1F9) are IL-1 family members that signal through the IL-1 receptor family members IL-1Rrp2 (IL-1RL2) and IL-1RAcP. IL-36gamma is secreted when transfected into 293-T cells and it could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1). Furthermore, IL-36gamma also can function as an agonist of NF-kappa B activation through the orphan IL-1- receptor-related protein 2. Human IL-36gamma (152a.a) shares 58 %, 59 %, 68 % and 69 % a.a. sequence identity with mouse, rat, bovine and equine IL-36gamma, respectively, and 23 - 57% a.a. sequence identity with other family members.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL36G
• Uniprot:
  • Q9NZH8()
  • Q9NZH8()
  • Q9NZH8()