IL-3 Antibody (OTI3B11)

Western Blot: IL-3 Antibody (3B11) [NBP1-47806] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL3.

Immunocytochemistry/Immunofluorescence: IL-3 Antibody (3B11) [NBP1-47806] - Staining of A549 cells using anti-IL3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IL-3 Antibody (3B11) [NBP1-47806] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue.

Immunohistochemistry-Paraffin: IL-3 Antibody (3B11) [NBP1-47806] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IL3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IL-3 Antibody (3B11) [NBP1-47806] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-IL3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IL-3 Antibody (3B11) [NBP1-47806] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IL3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IL-3 Antibody (3B11) [NBP1-47806] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-IL3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IL-3 Antibody (3B11) [NBP1-47806] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-IL3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IL-3 Antibody (3B11) [NBP1-47806] - Staining of paraffin-embedded Human breast tissue using anti-IL3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IL-3 Antibody (3B11) [NBP1-47806] - Staining of paraffin-embedded Human colon tissue using anti-IL3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IL-3 Antibody (3B11) [NBP1-47806] - Staining of paraffin-embedded Human Kidney tissue using anti-IL3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: IL-3 Antibody (3B11) [NBP1-47806] - Staining of paraffin-embedded Human breast tissue.

Product Discontinued

IL-3 Antibody (OTI3B11) Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 20-152 of human IL-3 (NP_000579) produced in E.coli.
• Specificity: This antibody is specific for Homo sapiens interleukin 3 (colony-stimulating factor, multiple) (IL3).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:500
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IL-3 Antibody (OTI3B11)

• Hematopoietic growth factor
• IL3
• IL-3
• IL-3MGC79398
• interleukin 3 (colony-stimulating factor, multiple)
• interleukin-3
• Mast cell growth factor
• mast-cell growth factor
• MCGF
• MCGFMGC79399
• MULTI-CSF
• multilineage-colony-stimulating factor
• Multipotential colony-stimulating factor
• P-cell stimulating factor
• P-cell-stimulating factor

Background

The protein encoded by IL-3 is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL3
• Entrez:
  • 3562(Human)