IL-29/IFN-lambda 1 Antibody - Azide and BSA Free

Western Blot: IL-29/IFN-lambda 1 Antibody [NBP1-77893] - Shows detection of recombinant human IL-29 raised in E.coli. Recombinant protein (0.1 ug, 19.9 kDa) was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Human IL-29. After washing, membrane was probed with secondary antibody DyLight (TM) 649 conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000 in blocking buffer for 30 min. at RT.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

IL-29/IFN-lambda 1 Antibody - Azide and BSA Free Summary

• Description: This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above
  
  Store this antibody at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This IgG fraction IL-29/IFN-lambda 1 Antibody was prepared from rabbit antiserum after repeated immunizations with mature length recombinant human IL-29/IFN-lambda 1/IFN-lambda 1 protein produced in E.coli. (Uniprot: Q8IU54)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IFNL1
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Western Blot 1:1000
• Application Notes: This purified antibody has been tested in western blotting and suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19.9 kDa in size corresponding to the mature human IL-29 protein by western blotting in appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with IL-29/IFN-lambda 1 from human sources based on 100% homology with the immunizing sequence. Based on high to 100% homology, there is a chance of cross-reactivity to IL-29/IFN-lambda 1 from a wide variety of animals. Cross-reactivity with IL-29/IFN-lambda 1 from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for IL-29/IFN-lambda 1 Antibody - Azide and BSA Free

• cytokine Zcyto21
• IFNL1
• IFN-lambda 1
• IFN-lambda-1
• IL29
• IL-29
• interferon lambda-1
• interferon, lambda 1
• interleukin 29 (interferon, lambda 1)
• interleukin-29
• ZCYTO21

Background

IL-29 (Interleukin-29) is a secreted cytokine with immunomodulatory activity. It may play a role in antiviral immunity. It up-regulates MHC class I antigen expression. IL-29 is a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Its induction is by viral infections or double stranded RNA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IFNL1
• Uniprot:
  • Q8IU54()
  • Q8IU54()
  • Q8IU54()
  • Q8IU54()
  • NP_742152.1(Human)