IL-28B/IFN-lambda 3 Antibody [Alexa Fluor® 594]

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 594

IL-28B/IFN-lambda 3 Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant mouse IL‑28B/IFN‑λ3
  Asp20-Val193
  Accession # Q8CGK6
• Specificity: Detects IL-28B/IFN-lambda 3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human IL-28A is observed. In Western blots, approximately 35% cross-reactivity (reducing conditions) and
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IFNL3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Neutralization
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-28B/IFN-lambda 3 Antibody [Alexa Fluor® 594]

• interleukin-28B
• IFN-lambda 3
• IL28B
• IL-28B
• IL28C
• interferon, lambda 3

Background

IL-28A, IL-28B, and IL-29, also named interferon-lambda 2 (IFN-lambda 2), IFN-lambda 3, and IFN-lambda 1, respectively, are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11-13% amino acid (aa) sequence identity) and the type I IFN family (15-19% aa sequence identity) (1-3). The genes encoding these three cytokines are localized to chromosome 19 and each is composed of multiple exons. The exon organization of these genes is also found in the IL-10 family genes but is distinct from the type I IFNs, which are encoded within a single exon. The expression of IL-28A, B, and IL-29 is induced by virus infection or double-stranded RNA. All three cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The three proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta ) and a novel IL-28 receptor alpha (IL-28 R alpha , also known as IFN-lambda R1). Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation. The phosphorylated STAT1 and STAT2 complex with IFN-regulatory factor 9 (IRF-9) to form the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex that is translocated to the nucleus. ISGF-3 binds to the IFN-stimulated response element (ISRE) present in the regulatory regions of the target genes. Mouse IL-28B cDNA encodes a 193 amino acid residue precursor protein with a putative 15 aa signal peptide. It shares 61%, 62%, and 52% aa sequence identity with human IL-28A, human IL-28B, and human IL-29, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IFNL3
• Uniprot:
  • Q8CGK6()