IL-28B/IFN-lambda 3 Antibody (567143)

Western blot shows lysates of human monocytes treated (+) with 10 ng/mL of rhIFN-alpha for 24 hours and then further stimulated with 10 µg/mL of poly I:C for 48 hours. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human IL-28B/IFN-lambda 3 Monoclonal Antibody (Catalog # MAB5259) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-28B/IFN-lambda 3 at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 567143
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-28B/IFN-lambda 3 Antibody (567143) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human IL‑28B/IFN‑ lambda 3
  Arg30-Val200 (Lys74Arg)
  Accession # AAN28264
• Specificity: Detects human IL-28B/IFN-lambda 3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-28A, rhIL-29, or recombinant mouse IL-28 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: IFNL3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-28B/IFN-lambda 3 Antibody (567143)

• interleukin-28B
• IFN-lambda 3
• IL28B
• IL-28B
• IL28C
• interferon, lambda 3

Background

IL-28B (also named interferon-lambda 3, IFN-lambda 3), IL-28A (IFN-lambda 2) and IL-29 (IFN-lambda 1) are type III interferons that are class II cytokine receptor ligands (1-4). They are distantly related to members of the IL-10 family and type I IFN family (1-4). Human IL-28B cDNA encodes a 200 amino acid (aa) protein with a 25 aa signal peptide and a 175 aa mature protein that lacks N-glycosylation sites. Mature human IL-28B shares 64% and 75% aa sequence identity with mouse and canine IL-28B, respectively, and is active across species (5). Human IL-28B shares 94% and 69% aa identity with human IL-28A and IL-29, respectively (4). Type III interferons are widely expressed, but are mainly produced by antigen presenting cells in response to viruses and double-stranded RNA that interact with Toll-like receptors or RIG-1 family helicases (2-6). They signal through a widely expressed receptor that is a heterodimer of the IL-10 receptor beta (IL-10 R beta ) and IL-28 receptor alpha (IL-28 R alpha ; also called IFN-lambda R1) (2, 3, 7, 9). Interaction of either type I or type III IFNs with their receptors activates similar pathways, including JAK tyrosine kinase activation, STAT phosphorylation and formation of the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex (1-3). Both type I and III IFNs induce anti-viral activity and upregulate MHC class I antigen expression (2-6). Cell lines responsive to type III IFNs are also responsive to type I IFNs, but in general, higher concentrations of type III IFNs are needed for similar in vitro responses (8). In vivo, however, type III IFNs enhance levels of IFN-gamma in serum, suggesting that the robust anti-viral activity of type III IFNs may stem in part from activation of the immune system (5, 7). Anti-proliferative and antitumor activity in vivo has also been shown for type III IFNs (9-11).

1. Chen, Q. et al. (2006) Vitam. Horm. 74:207.
2. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
3. Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
4. Bartlett, N.W. et al. (2005) J. Gen. Virol. 86:1589.
5. Ank, N. et al. (2006) J. Virol. 80:4501.
6. Onoguchi, K. et al. (2007) J. Biol. Chem. 282:7576.
7. Siebler, J. et al. (2007) Gastroenterology 132:358.
8. Meager, A. et al. (2005) Cytokine 31:109.
9. Lasfar, A. et al. (2006) Cancer Res. 66:4468.
10. Sato, A. et al. (2006) J. Immunol. 176:7686.
11. Zitzmann, K. et al. (2006) Biochem. Biophys. Res. Commun. 344:1334.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-28B/IFN-lambda 3 Antibody (MAB5259)(4)

Publications using MAB5259

• Kanai, M;Sugiyama, M;Kondo, M;Yamada, K;Nishimura, M;Mano, S; Fusing the 3'UTR of seed storage protein genes leads to massive recombinant protein accumulation in seeds Scientific reports 2023-07-27 [PMID: 37500719] (Western Blot, Plant - Arabidopsis thaliana)
• CA Smith, DJ Tyrell, UA Kulkarni, S Wood, L Leng, RL Zemans, R Bucala, DR Goldstein Macrophage migration inhibitory factor enhances influenza-associated mortality in mice JCI Insight, 2019-07-11;4(13):. 2019-07-11 [PMID: 31292300] (Western Blot, Human)
• J Corry, N Arora, CA Good, Y Sadovsky, CB Coyne Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface Proc. Natl. Acad. Sci. U.S.A., 2017-08-07;0(0):. 2017-08-07 [PMID: 28784796] (Neutralization, Human)
• Stone , Amy E L, Giugliano , Silvia, Schnell , Gretja, Cheng , Linling, Leahy , Katelyn, Golden-Mason , Lucy, Gale , Michael, Rosen , Hugo R Hepatitis C virus pathogen associated molecular pattern (PAMP) triggers production of lambda-interferons by human plasmacytoid dendritic cells. PLoS Pathog, 2013-04-18;9(4):e1003316. 2013-04-18 [PMID: 23637605] (Western Blot, Human)

Bioinformatics

• Gene Symbol: IFNL3
• Uniprot:
  • AAN28264()