IL-28A/IFN-lambda 2 Antibody [Biotin]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

IL-28A/IFN-lambda 2 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL‑28A/IFN‑ lambda 2
  Asp20-Val193
  Accession # NP_001019844
• Specificity: Detects mouse IL‑28A/IFN‑ lambda 2 in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse IL-28B is observed, less than 10% cross-reactivity with recombinant human (rh) IL-28B is observed, and less than 5% cross-reactivity with rhIL-28A is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: IFNL2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-28A/IFN-lambda 2 Antibody [Biotin]

• interleukin-28A
• IFN-lambda 2
• IL28A
• IL-28A
• interferon, lambda 2

Background

IL-28A (also named interferon-lambda 2, IFN-lambda 2), IL-28B (IFN-lambda 3) and IL-29 (IFN-lambda 1) are type III interferons that are class II cytokine receptor ligands (1‑4). They are distantly related to members of the IL-10 family and type I IFN family (1‑4). Mouse IL-28A cDNA encodes a 193 amino acid (aa) protein with a 19 aa signal peptide and a 174 aa mature protein that lacks N-glycosylation sites. Mature mouse IL-28A shares 81% and 66% aa sequence identity with rat and human IL-28A, respectively, and functions across species (5). Mouse IL-28A and IL-28B share 97% aa identity; the mouse lacks a functional IL-29 gene (4). Type III interferons are widely expressed, but are mainly produced by antigen presenting cells in response to viruses and double-stranded RNA that interact with Toll-like receptors or RIG-1 family helicases (2‑6). They signal through a widely expressed receptor that is a heterodimer of the IL-10 receptor  beta (IL-10 R beta ) and IL-28 receptor  alpha (IL-28 R alpha ; also called IFN-lambda  R1) (2, 3, 7, 9). Interaction of either type I or type III IFNs with their receptors activates similar pathways, including JAK tyrosine kinase activation, STAT phosphorylation and formation of the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex (1‑3). Both type I and III IFNs induce antiviral activity and upregulate MHC class I antigen expression (2‑6). Cell lines responsive to type III IFNs are also responsive to type I IFNs, but in general, higher concentrations of type III IFNs are needed for similar in vitro responses (8). In vivo, however, type III IFNs enhance levels of IFN-gamma in serum, suggesting that the robust antiviral activity of type III IFNs may stem in part from activation of the immune system (5, 7). Anti-proliferative and antitumor activity in vivo has also been shown for type III IFNs (9‑11).

1. Chen, Q. et al. (2006) Vitam. Horm. 74:207.
2. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
3. Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
4. Bartlett, N.W. et al. (2005) J. Gen. Virol. 86:1589.
5. Ank, N. et al. (2006) J. Virol. 80:4501.
6. Onoguchi, K. et al. (2007) J. Biol. Chem. 282:7576.
7. Siebler, J. et al. (2007) Gastroenterology 132:358.
8. Meager, A. et al. (2005) Cytokine 31:109.
9. Lasfar, A. et al. (2006) Cancer Res. 66:4468.
10. Sato, A. et al. (2006) J. Immunol. 176:7686.
11. Zitzmann, K. et al. (2006) Biochem. Biophys. Res. Commun. 344:1334.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-28A/IFN-lambda 2 Antibody (BAF4635)(1)

Publication using BAF4635

• Iversen MB, Ank N, Melchjorsen J, Paludan SR Expression of type III interferon (IFN) in the vaginal mucosa is mediated primarily by dendritic cells and displays stronger dependence on NF-kappaB than type I IFNs. J. Virol., 2010-02-24;84(9):4579-86. 2010-02-24 [PMID: 20181703] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: IFNL2
• Uniprot:
  • NP_001019844()