IL-22BP/IL22 RA2 Antibody

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-22BP/IL22 RA2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL‑22BP
  Thr19-Pro230 (Asp149Glu)
  Accession # Q80XF5
• Specificity: Detects mouse IL-22BP in direct ELISAs and Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) IL-22BP is observed and approximately 5% cross-reactivity with rhIL-22 R is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: IL22RA2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-22BP/IL22 RA2 Antibody

• class II cytokine receptor
• CRF2-10
• CRF2-S1IL22BP
• CRF2-X
• Cytokine receptor class-II member 10
• Cytokine receptor family 2 member 10
• Cytokine receptor family type 2, soluble 1
• IL-22 RA2
• IL-22 receptor subunit alpha-2
• IL22BP
• IL-22BP
• IL-22BPCRF2X
• IL22RA2
• IL-22RA2
• IL-22R-alpha-2
• interleukin 22 receptor, alpha 2
• interleukin 22-binding protein
• interleukin-22 receptor subunit alpha-2
• Interleukin-22-binding protein
• MGC150509
• MGC150510
• zcytoR16

Background

Interleukin 22 binding protein (IL-22BP), also known as CRF2-10, CRF2-X, and IL-22 RA2, is a 35‑45 kDa secreted glycoprotein belonging to the type II cytokine receptor family (CRF). IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta . IL-10 R beta is also a component of the receptor complexes for IL‑10, IL-26, IL-28, and IL-29 (1‑4). IL‑10 R beta is widely expressed, and other cell types express either IL-10 R alpha or IL-22 R but not both (5). The mouse IL-22BP cDNA encodes a 230 amino acid (aa) precursor that includes a 20 aa signal sequence and two fibronectin type-III domains (6). Mouse IL-22BP shares 59% and 85% aa sequence identity with human and rat IL-22BP, respectively. Among type II cytokine receptors, IL-22BP is most closely related to the extracellular domain of IL-22 R, sharing 36% aa sequence identity. Splice variants of human IL-22BP exist but have not been detected for the mouse protein (6‑8). IL-22BP binds an epitope on IL-22 that is required for the interaction of IL-22 with the transmembrane IL-22 R (8‑10), thereby preventing the association of IL-22 R with IL-10 R beta (10). IL-22BP blocks IL‑22‑induced responses including induction of MHC class I expression, Stat1 and Stat3 activation, SOCS3 gene expression,the production of reactive oxygen species (6, 9) and the modulation of the proinflammatory effects of IL-22 (11). Mouse IL-22 BP neutralizes both mouse and human IL-22 (6). IL-22BP is secreted by epithelial cells, dendritic cells, activated B cells, and activated monocytes (6, 8, 12, 13).

1. Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.
2. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
3. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
4. Kotenko, S.V. (2002) Cytokine Growth Factor Rev. 13:223.
5. Wolk, K. et al. (2005) Genes Immun. 6:8.
6. Wei, C.-C. et al. (2003) Genes Immun. 4:204.
7. Dumoutier, L. et al. (2001) J. Immunol. 166:7090.
8. Xu, W. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9511.
9. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
10. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
11. Whittington, H.A. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:220.
12. Lecart, S. et al. (2002) Int. Immunol. 14:1351.
13. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-22BP/IL22 RA2 Antibody (AF2376)(3)

Publications using AF2376

• Tayyab, M;Minkkinen, MME;Stenvall, CA;Polari, L;Nielsen, V;Shah, YM;Toivola, DM; Genetically Induced Mouse Model for Colon-specific Epithelial Cell Tumorigenesis Driven by Loss of K8 and Apc Cellular and molecular gastroenterology and hepatology 2025-12-24 [PMID: 41453638] (Western Blot, Mouse)
• C Tang, H Sun, M Kadoki, W Han, X Ye, Y Makusheva, J Deng, B Feng, D Qiu, Y Tan, X Wang, Z Guo, C Huang, S Peng, M Chen, Y Adachi, N Ohno, S Trombetta, Y Iwakura Blocking Dectin-1 prevents colorectal tumorigenesis by suppressing prostaglandin E2 production in myeloid-derived suppressor cells and enhancing IL-22 binding protein expression Nature Communications, 2023-03-17;14(1):1493. 3/17/2023 [PMID: 36932082]
• W Li, A Dick, F Lu, H Zhang, H Sun Induction of MET Receptor Tyrosine Kinase Down-regulation through Antibody-mediated Receptor Clustering Sci Rep, 2019-02-13;9(1):1988. 2019-02-13 [PMID: 30760737] (Western Blot, Human)

Bioinformatics

• Gene Symbol: IL22RA2
• Uniprot:
  • Q80XF5(Sheep)