IL-22BP/IL22 RA2 Antibody [Alexa Fluor® 488]

• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

IL-22BP/IL22 RA2 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL‑22BP
  Thr19-Pro230 (Asp149Glu)
  Accession # Q80XF5
• Specificity: Detects mouse IL-22BP in direct ELISAs and Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) IL-22BP is observed and approximately 5% cross-reactivity with rhIL-22 R is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Interleukin 22 binding protein (IL-22BP), also known as CRF2-10, CRF2-X, and IL-22 RA2, is a 35‑45 kDa secreted glycoprotein belonging to the type II cytokine receptor family (CRF). IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta . IL-10 R beta is also a component of the receptor complexes for IL‑10, IL-26, IL-28, and IL-29 (1‑4). IL‑10 R beta is widely expressed, and other cell types express either IL-10 R alpha or IL-22 R but not both (5). The mouse IL-22BP cDNA encodes a 230 amino acid (aa) precursor that includes a 20 aa signal sequence and two fibronectin type-III domains (6). Mouse IL-22BP shares 59% and 85% aa sequence identity with human and rat IL-22BP, respectively. Among type II cytokine receptors, IL-22BP is most closely related to the extracellular domain of IL-22 R, sharing 36% aa sequence identity. Splice variants of human IL-22BP exist but have not been detected for the mouse protein (6‑8). IL-22BP binds an epitope on IL-22 that is required for the interaction of IL-22 with the transmembrane IL-22 R (8‑10), thereby preventing the association of IL-22 R with IL-10 R beta (10). IL-22BP blocks IL‑22‑induced responses including induction of MHC class I expression, Stat1 and Stat3 activation, SOCS3 gene expression,the production of reactive oxygen species (6, 9) and the modulation of the proinflammatory effects of IL-22 (11). Mouse IL-22 BP neutralizes both mouse and human IL-22 (6). IL-22BP is secreted by epithelial cells, dendritic cells, activated B cells, and activated monocytes (6, 8, 12, 13).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.