IL-20 R beta/FNDC6 Antibody

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-20 R beta/FNDC6 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL-20 R beta
  Asp30-Ala230
  Accession # Q6UXL0
• Specificity: Detects human IL-20 R beta in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse IL-20 R beta is observed and less than 2% cross-reactivity with recombinant human (rh) IL-20 R alpha , rhIL-10 R beta , rhIFN-gamma R1, and rhIFN-gamma R2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL20RB
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-20 R beta/FNDC6 Antibody

• FNDC6
• DIRS1
• FNDC6
• IL-20 R beta
• IL20R beta
• IL-20R2
• IL20RB interleukin 20 receptor beta
• IL20RB
• IL-20Rb
• MGC34923

Background

IL-20 receptor beta (IL-20 R beta ), also known as IL-20 R2, is a transmembrane glycoprotein in the class II cytokine receptor family. These receptors are characterized by tandem fibronectin type III domains in their extracellular region and the lack of a WSXWS motif (1). Class II cytokine receptors form heterodimeric signaling receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1). Human IL-20 R beta consists of a 204 aa extracellular domain (ECD) with two fibronectin type III domains, a 21 aa transmembrane segment, and a 57 aa cytoplasmic domain (2). Within the ECD, human IL-20 R beta shares 81% aa sequence identity with mouse IL-20 R beta . It shares 15%‑28% aa sequence identity with the human class II cytokine receptors IFN‑ alpha  R1, IFN-alpha R2, IFN-gamma R1, IFN-gamma R2, IL-10 R alpha , IL-10 R beta , IL-20 R alpha , IL-22BP, IL-22 R alpha , IL-28 R, and tissue factor. Strong IL-20 R beta expression is normally restricted to skin and testis (3). IL-20 R beta is also expressed in psoriatic skin, rheumatoid arthritis synovial membranes, and hepatocytes of LPS-treated mice, and it contributes to the local inflammatory reaction (3‑6). IL-20 R beta heterodimerizes with IL-20 R alpha to form the receptor complex that mediates IL-19, IL-20 and IL-24 signals (3, 7‑10). It also heterodimerizes with IL-22 R to form the functional receptor complex for IL-20 and IL-24 (7‑9). Binding of these IL-10 family class II cytokines to their receptors induces activation of the JAK-STAT signal transduction pathway.

1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
2. SwissProt # Q6UXL0.
3. Blumberg, H. et al. (2001) Cell 104:9.
4. Hsu, Y.-H. et al. (2006) Arthritis Rheum. 54:2722.
5. Wegenka, U.M. et al. (2007) J. Hepatol. 46:257.
6. Chan, J.R. et al. (2006) J. Exp. Med. 203:2577.
7. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517.
8. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
9. Wang, M., et al. (2002) J. Biol. Chem. 277:7341.
10. Pletnev, S. et al. (2003) Biochemistry 42:12617.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-20 R beta/FNDC6 Antibody (AF1788)(7)

Publications using AF1788

• Jacek Lubkowski, Cem Sonmez, Sergey V. Smirnov, Andriy Anishkin, Sergei V. Kotenko, Alexander Wlodawer Crystal structure of the labile complex of interleukin 24 (IL-24) with the extracellular domains of IL-22R1 and IL-20R2 The Journal of Immunology 10/1/2018 [PMID: 30111632]
• QUNFENG MA, BANGMING JIN, YAO ZHANG, YINAN SHI, CHI ZHANG, DAN LUO et al. Secreted recombinant human IL-24 protein inhibits the proliferation of esophageal squamous cell carcinoma Eca-109 cells in vitro and in vivo Oncology Reports 5/1/2016 [PMID: 26935394]
• QUNFENG MA, BANGMING JIN, YAO ZHANG, YINAN SHI, CHI ZHANG, DAN LUO et al. Secreted recombinant human IL-24 protein inhibits the proliferation of esophageal squamous cell carcinoma Eca-109 cells in vitro and in vivo Oncology Reports 2016-05-01 [PMID: 26935394] (Neutralization, Immunocytochemistry, Human)
• QUNFENG MA, XUEFENG DENG, BANGMING JIN, YAO ZHANG, DAN LUO, HEYU SONG et al. A novel human interleukin-24 peptide created by computer-guided design contributes to suppression of proliferation in esophageal squamous cell carcinoma Eca-109 cells Oncology Reports 1/1/2015 [PMID: 25371158]
• QUNFENG MA, XUEFENG DENG, BANGMING JIN, YAO ZHANG, DAN LUO, HEYU SONG et al. A novel human interleukin-24 peptide created by computer-guided design contributes to suppression of proliferation in esophageal squamous cell carcinoma Eca-109 cells Oncology Reports 2015-01-01 [PMID: 25371158] (Immunocytochemistry, Human)
• Dash R, Bhoopathi P, Das S, Sarkar S, Emdad L, Dasgupta S, Sarkar D, Fisher P Novel mechanism of MDA-7/IL-24 cancer-specific apoptosis through SARI induction. Cancer Res, 2013-11-26;74(2):563-74. 2013-11-26 [PMID: 24282278] (Western Blot, Human)
• Tohyama M, Hanakawa Y, Shirakata Y, Dai X, Yang L, Hirakawa S, Tokumaru S, Okazaki H, Sayama K, Hashimoto K IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression. Eur. J. Immunol., 2009-10-01;39(10):2779-88. 2009-10-01 [PMID: 19731362] (Western Blot, Human)

Bioinformatics

• Gene Symbol: IL20RB
• Uniprot:
  • Q6UXL0()