IL-20 Antibody (287023)

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 287023
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-20 Antibody (287023) Summary

• Immunogen: E. coli-derived recombinant human IL‑20
  Leu25-Glu176
  Accession # AAF36679
• Specificity: Detects human and mouse IL-20 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL20
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-20 Antibody (287023)

• Cytokine Zcyto10
• IL10D
• IL20
• IL-20
• IL-20ZCYTO10four alpha helix cytokine
• interleukin 20
• interleukin-20
• MGC96907

Background

Human Interleukin 20 (IL-20) was identified by searching sequence databases for translated sequences containing a signal sequence and amphipathic helices found in helical cytokines. Human IL-20 is synthesized as a 176 amino acid (aa) precursor with a 24 aa signal sequence and a 152 aa mature segment. There are no N-linked glycosylation sites and it is doubtful that the native molecule is glycosylated. Although IL-20 is a distant member of the IL-10 family, it functions as a monomer. IL‑20 shares less than 40% aa sequence identity with other IL-10 family members. Mouse and human IL-20 share 77% aa sequence identity in their mature segments. Human IL-20 is active on mouse cells. IL-20 production has been found in skin and trachea. In particular, activated keratinocytes and, possibly, monocytes are reported to express IL-20. There are two heterodimeric receptor complexes for IL-20. The first is composed of IL‑20 R alpha and IL‑20 R beta . The second is composed of IL‑22 R and IL‑20 R beta . Whereas the IL-22 R/IL-20 R beta complex is shared with IL-24/mda-7, the IL-20 R alpha /IL-20 R beta  complex is shared with both IL-19 and IL-24. Little is known about the function of IL-20. It is reported to induce the proliferation of multipotential hematopoietic progenitor cells, direct the differentiation and expansion of keratinocytes, and promote the release of proinflammatory mediators in keratinocytes and other IL-20 receptor expressing cells (1‑6).

1. Blumberg, H. et al. (2001) Cell 104:9.
2. Liu, L. et al. (2003) Blood 102:3206. 
3. Rich, B.E. and T.S. Kupper (2001) Curr. Biol. 11:R531.
4. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. 
5. Dumoutier, L. (2001) J. Immunol. 167:3545. 
6. Romer, J. (2003) J. Invest. Dermatol. 121:1306.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-20 Antibody (MAB11021)(1)

Publication using MAB11021

• Wei CC, Chang MS A novel transcript of mouse interleukin-20 receptor acts on glomerular mesangial cells as an aggravating factor in lupus nephritis. Genes Immun., 2008-09-04;9(8):668-79. 2008-09-04 [PMID: 18769441] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: IL20
• Uniprot:
  • AAF36679()