IL-1Rrp2/IL-1R6 Antibody [Alexa Fluor® 350]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 350

IL-1Rrp2/IL-1R6 Antibody [Alexa Fluor® 350] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL‑1 Rrp2/IL‑1 R6
  Asp22-Arg338 (Ala158Val)
  Accession # Q9ERS7
• Specificity: Detects mouse IL‑1 Rrp2/IL‑1 R6 in direct ELISAs and Western blots. In direct ELISAs, less than 60% with recombinant rat IL-1 Rrp2 and recombinant human IL-1 Rrp2 is observed and less than 5% cross‑reactivity with recombinant mouse (rm) IL-1 R4 is ob
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1Rrp2/IL-1R6 Antibody [Alexa Fluor® 350]

• IL-1 R6
• IL-1 Rrp2
• IL1RL2
• IL-1RL2
• IL1Rrp2
• IL-1Rrp2
• IL1RRP2IL1R-rp2Interleukin-1 receptor-related protein 2
• interleukin 1 receptor-like 2
• interleukin-1 receptor-like 2

Background

IL-1 Rrp2, also known as IL-1 R6, is a member of the IL-1 R/TLR superfamily. Members of this superfamily have a characteristic Toll-IL-1 R (TIR) domain in their cytoplasmic region. This superfamily can be further divided into three subgroups based on extracellular region analogies. IL-1 Rrp2 belongs to Subgroup 1 which is characterized by the presence of at least one immunoglobulin-like domain (1). Mouse IL-1 Rrp2/IL-1R6 is a 70 kDa type I transmembrane glycoprotein that is synthesized as a 574 amino acid (aa) precursor. The precursor contains a 21 aa signal sequence, a 317 aa extracellular region, a 21 aa transmembrane segment, and a 215 aa cytoplasmic domain (2). The extracellular region contains three C-type Ig-like domains. Within the extracellular region, mouse IL-1 Rrp2 is 64% and 80% aa identical to human and rat IL-1 Rrp2, respectively. Expression of IL-1 Rrp2 is restricted to lung epithelium, monocytes, skin-derived keratinocytes, fibroblasts and, to a lesser extent, endothelial cells (3). Studies have demonstrated that the functional receptor for IL-1F6, IL-1F8 and IL-1F9 is a combination of IL-1 Rrp2 and IL‑1 RAcP. This suggests that IL-1 Rrp2 ligands may signal in a similar fashion to IL-1 and IL-18. In each case, there is a binding receptor which, upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.