Recombinant Human IL-19 Protein

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human IL-19 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 153 amino acids corresponding to IL-19 Source: E. coli

  Uniprot ID: Q9UHD0

  Amino Acid Sequence: LRRCLISTDM HHIEESFQEI KRAIQAKDTF PNVTILSTLE TLQIIKPLDV CCVTKNLLAF YVDRVFKDHQ EPNPKILRKI SSIANSFLYM QKTLRQCQEQ RQCHCRQEAT NATRVIHDNY DQLEVHAAAI KSLGELDVFL AWINKNHEVM SSA

• Details of Functionality: IL-19 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-20R alpha and human IL-20R beta co-transfected murine BaF3 pro-B cells is less than 1.5 ng/ml, corresponding to a specific activity of > 6.7 x 10^5 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL19
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of IL-19 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 17.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in 100 mM HAC to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human IL-19 Protein

• IL-10C
• IL19
• IL-19
• interleukin 19
• MDA1
• melanoma differentiation associated protein-like protein
• NG.1Melanoma differentiation-associated protein-like protein
• ZMDA1interleukin-19

Background

Human Interleukin-19 (IL-19) is encoded by the IL19 gene, which is located on the chromosome 1. The protein belongs to the IL-10 family that includes IL-10, IL-20, IL-22, IL-24, and IL-26. As a monomer made of seven amphipathic helices, IL-19 has a helical bundle and shares the same cell surface receptor (IL-20R) with IL-20 and IL-24. It may play some important roles in inflammatory responses because it up-regulates IL-6 and TNF-alpha and induces apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL19
• Uniprot:
  • Q9UHD0()
  • Q9UHD0()
  • Q9UHD0()