IL-19 Overexpression Lysate (Native)


Western Blot: IL19 Overexpression Lysate (Adult Normal) [NBL1-11921] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL19.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

IL-19 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC212571 and based on accession number NM_013371. The protein contains a C-terminal DDK tag.
Homo sapiens interleukin 19 (IL19), transcript variant 2, mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
17.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for IL-19

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for IL-19 Overexpression Lysate (Native)

  • IL-10C
  • IL19
  • IL-19
  • interleukin 19
  • MDA1
  • melanoma differentiation associated protein-like protein
  • NG.1Melanoma differentiation-associated protein-like protein
  • ZMDA1interleukin-19


The protein encoded by this gene is a cytokine that belongs to the IL10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Alternatively spliced transcript variants encoding the distinct isoforms have been described. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' end-region, which introduces an in-frame stop codon, as compared to variant 1. The resulting isoform (2) has a shorter N-terminus, as compared to isform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Gene Symbol IL19