IL-19 Overexpression Lysate (Native)

Images

 
Western Blot: IL19 Overexpression Lysate (Adult Normal) [NBL1-11921] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL19.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

IL-19 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC212571 and based on accession number NM_013371. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens interleukin 19 (IL19), transcript variant 2, mRNA.
Gene
IL19

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
17.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for IL-19

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for IL-19 Overexpression Lysate (Native)

  • IL-10C
  • IL19
  • IL-19
  • interleukin 19
  • MDA1
  • melanoma differentiation associated protein-like protein
  • NG.1Melanoma differentiation-associated protein-like protein
  • ZMDA1interleukin-19

Background

The protein encoded by this gene is a cytokine that belongs to the IL10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Alternatively spliced transcript variants encoding the distinct isoforms have been described. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' end-region, which introduces an in-frame stop codon, as compared to variant 1. The resulting isoform (2) has a shorter N-terminus, as compared to isform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF1709
Species: Mu
Applications: WB, Block, CyTOF-ready, ELISA(Cap), ELISA(Det), ICFlow, ELISA(Sta)
1102-IL/CF
Species: Hu
2915-IL/CF
Species: Mu
AF1375
Species: Hu
Applications: WB
1965-IL/CF
Species: Hu
MAB15041
Species: Hu
Applications: WB, IHC, CyTOF-ready, ICFlow
NB100-56409
Species: Hu, Mu
Applications: WB, Flow
NBP2-22471
Species: Hu, Mu, Rt, Mk, Pm
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
MAB274
Species: Hu
Applications: Neut

Publications for IL-19 Lysate (NBL1-11921) (0)

There are no publications for IL-19 Lysate (NBL1-11921).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IL-19 Lysate (NBL1-11921) (0)

There are no reviews for IL-19 Lysate (NBL1-11921). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-19 Lysate (NBL1-11921) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IL-19 Products

Bioinformatics Tool for IL-19 Lysate (NBL1-11921)

Discover related pathways, diseases and genes to IL-19 Lysate (NBL1-11921). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-19 Lysate (NBL1-11921)

Discover more about diseases related to IL-19 Lysate (NBL1-11921).
 

Pathways for IL-19 Lysate (NBL1-11921)

View related products by pathway.

PTMs for IL-19 Lysate (NBL1-11921)

Learn more about PTMs related to IL-19 Lysate (NBL1-11921).
 

Research Areas for IL-19 Lysate (NBL1-11921)

Find related products by research area.

Blogs on IL-19

There are no specific blogs for IL-19, but you can read our latest blog posts.

Customers Who Bought This Also Bought

IL-19
2915-IL-025/CF
IL-4 [Unconjugated]
204-IL-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our IL-19 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol IL19
Entrez