Human IL-13R alpha 2 ELISA Kit (Colorimetric) Summary
| Description |
Incubation: 3 hours 45 minutes |
| Specificity |
Recognizes both natural and recombinant IL-13R alpha 2. No cross reactivity has been observed with TNF-a, TRAIL, IFN-g, Fas, IL-1a, IL-4, IL-6, IL-8, IL-13, Granzyme B. |
| Standard Curve Range |
156-5000 pg/ml |
| Sensitivity |
150 pg/ml |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 6.06 |
| Intra-Assay |
CV% < 3.10 |
| Spike Recovery |
79 to 100% |
| Sample Volume |
100 ul |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
IL13RA2 |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze. |
Kit Components
|
Components
|
- Biotinylated secondary antibody
- Buffers
- Controls (where applicable)
- Detailed procedure.
- Pre-coated strip plates
- Standards
- Stop reagents
- Streptavidin-HRP
- TMB
|
Alternate Names for Human IL-13R alpha 2 ELISA Kit (Colorimetric)
Background
IL13 receptor alpha 2 is encoded by this gene is closely related to Il13RA1, a subuint of the interleukin 13 receptor complex. This protein binds IL13 with high affinity, but lacks cytoplasmic domain, and does not appear to function as a signal mediator. It is reported to play a role in the internalization of IL13.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for IL-13R alpha 2 ELISA Kit (NBP3-14632). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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