IL-13R alpha 1 Antibody (419718) [Phycoerythrin]

Human peripheral blood granulocytes were stained with (A) Mouse Anti-Human Siglec-3/CD33 APC-conjugated Monoclonal Antibody (Catalog # FAB1137A) and (B) Mouse Anti-Human IL-13 Ra1 PE-conjugated Monoclonal Antibody (Catalog # FAB1462P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 419718
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

IL-13R alpha 1 Antibody (419718) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL‑13 R alpha 1
  Ala27-Thr343
  Accession # P78552
• Specificity: Detects human IL-13 R alpha 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL-13 R alpha 1, recombinant human (rh) IL-13 R alpha 2, rhIL-4 R, rhIL-5 R beta , or rhIL-9 R is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL13RA1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-13R alpha 1 Antibody (419718) [Phycoerythrin]

• bB128O4.2.1 (interleukin 13 receptor, alpha 1)
• Cancer/testis antigen 19
• CD213a1 antigen
• CD213a1
• CT19
• IL-13 R alpha 1
• IL13 receptor alpha-1 chain
• IL-13 receptor subunit alpha-1
• IL13R alpha 1
• IL-13R subunit alpha-1
• IL13R
• IL13RA
• IL-13Ra
• IL13RA1
• IL-13Ra1
• IL-13R-alpha-1
• interleukin 13 receptor, alpha 1
• interleukin-13 receptor subunit alpha-1
• NR4

Background

Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha , IL-13 R alpha or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.

The human IL-13 R alpha 1 was originally cloned based on sequence homology to the mouse IL-13 R alpha 1. The IL-13 R alpha 1 cDNA encodes a 427 amino acid (aa) precursor protein with a putative 21 aa signal peptide, a 324 aa extracellular domain, a 23 aa transmembrane region and a 59 aa cytoplasmic tail. Human and mouse IL-13 R alpha 1 share 76% aa sequence identity. The extracellular domain of IL-13 R alpha 1 is also closely related to that of IL-13 R alpha 2. IL‑13 R alpha 1 has been shown to combine with the IL-4 R alpha to form a high-affinity receptor complex capable of transducing an IL-13-dependent proliferative signal. The role of IL-13 R alpha 2 in IL-13 signaling remains to be elucidated.

1. Caput, D. et al. (1996) J. Biol. Chem. 271:16921.
2. Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
3. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
4. Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. USA 93:497.
5. Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-13R alpha 1 Antibody (FAB1462P)(2)

Publications using FAB1462P

• S Trabanelli, MF Chevalier, A Martinez-U, A Gomez-Cade, B Salomé, M Lecciso, V Salvestrin, G Verdeil, J Racle, C Papayannid, H Morita, I Pizzitola, C Grandcléme, P Bohner, E Bruni, M Girotra, R Pallavi, P Falvo, EO Leibundgut, GM Baerlocher, C Carlo-Stel, D Taurino, A Santoro, O Spinelli, A Rambaldi, E Giarin, G Basso, C Tresoldi, F Ciceri, D Gfeller, CA Akdis, L Mazzarella, S Minucci, PG Pelicci, E Marcenaro, ANJ McKenzie, D Vanhecke, G Coukos, D Mavilio, A Curti, L Derré, C Jandus Tumour-derived PGD2 and NKp30-B7H6 engagement drives an immunosuppressive ILC2-MDSC axis Nat Commun, 2017-09-19;8(1):593. 2017-09-19 [PMID: 28928446] (Flow Cytometry, Human)
• Kong S, Sengupta S, Tyler B, Bais A, Ma Q, Doucette S, Zhou J, Sahin A, Carter B, Brem H, Junghans R, Sampath P Suppression of human glioma xenografts with second-generation IL13R-specific chimeric antigen receptor-modified T cells. Clin Cancer Res, 2012-09-10;18(21):5949-60. 2012-09-10 [PMID: 22966020] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL13RA1
• Uniprot:
  • P78552()