IL-12 R beta 1 Antibody (69310) [Allophycocyanin]

Daudi cells were stained with Mouse Anti-Human IL‑12 R beta 1 APC‑conjugated Monoclonal Antibody (Catalog # FAB839A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

PBMC cells treated with 5ug/ml PHA (Sigma) for 48-72 hours were stained with Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human IL‑12 R beta 1 APC‑conjugated Monoclonal Antibody (Catalog # FAB839A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 69310
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

IL-12 R beta 1 Antibody (69310) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL-12 R beta 1
  Cys24-Glu540
  Accession # P42701
• Specificity: Detects human IL-12 R beta 1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-12 R beta 1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL12RB1
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  12 months from date of receipt, 2 to 8 degreesC as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12 R beta 1 Antibody (69310) [Allophycocyanin]

• CD212 antigen
• CD212
• IL-12 R beta 1
• IL-12 receptor beta component
• IL-12 receptor subunit beta-1
• IL12R beta 1
• IL-12R subunit beta-1
• IL12R
• IL12RB
• IL12RB1
• IL-12Rb1
• IL-12R-BETA1
• IL-12R-beta-1
• interleukin 12 receptor, beta 1
• interleukin-12 receptor beta-1 chain
• interleukin-12 receptor subunit beta-1
• MGC34454

Background

Interleukin12 (IL-12) is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of two subunits designated IL-12 R beta 1 and IL-12 R beta 2. Individually, IL-12 R beta 1 and IL-12 R beta 2 bind IL-12 with low affinity. Co-expression of both subunits confers high-affinity binding and is required for IL-12 activity. Both IL-12 receptor subunits are type I transmembrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta 1 cDNA encodes a 662 amino acid (aa) protein with a putative 23 aa signal peptide that is cleaved to generate the mature protein with a 522 aa extracellular domain, a 25 aa transmembrane domain and a 92 aa cytoplasmic region. Expression of IL-12 R beta 1 is detected in activated T cells, NK cells and B cells. The expression of IL-12 R beta 2 is more restricted and appears to be limited to Th2 cells.

1. Gately, M.K. et al. (1998) Annu. Rev. Immunol. 16:495.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL12RB1
• Uniprot:
  • P42701()