Ikaros/IKZF1 Antibody (JB50-38)

Western Blot: Ikaros/IKZF1 Antibody (JB50-38) [NBP2-75536] - Analysis of Ikaros on human thymus tissue lysate using anti-Ikaros antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody (JB50-38) [NBP2-75536] - Analysis of paraffin-embedded mouse spleen tissue using anti-Ikaros antibody. Counter stained with hematoxylin.

Flow Cytometry: Ikaros/IKZF1 Antibody (JB50-38) [NBP2-75536] - Analysis of Daudi cells with Ikaros antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody (JB50-38) [NBP2-75536] - Analysis of paraffin-embedded human spleen tissue using anti-Ikaros antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody (JB50-38) [NBP2-75536] - Analysis of paraffin-embedded human tonsil tissue using anti-Ikaros antibody. Counter stained with hematoxylin.

Product Discontinued

Ikaros/IKZF1 Antibody (JB50-38) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Ikaros/IKZF1 aa 261-353 / 519. (SwissProt: Q13422 Human)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IKZF1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Ikaros/IKZF1 Antibody (JB50-38)

• DNA-binding protein Ikaros
• hIk-1
• IK1
• IK1LyF-1
• Ikaros (zinc finger protein)
• IKAROS family zinc finger 1 (Ikaros)
• Ikaros family zinc finger protein 1
• Ikaros
• IKAROSLymphoid transcription factor LyF-1
• IKZF1
• LYF1
• LyF-1
• LYF1PRO0758
• PRO0758
• zinc finger protein, subfamily 1A, 1 (Ikaros)
• ZNFN1A1
• ZNFN1A1CLL-associated antigen KW-6

Background

Transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Binds with higher affinity to gamma satellite A. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (terminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IKZF1