Mouse IgM Isotype Control [FITC]

• Reactivity: Mu
• Applications: WB
• Host: Mouse
• Conjugate: FITC
• Concentration: LYOPH

Mouse IgM Isotype Control [FITC] Summary

• Description: Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Mouse Serum and anti-Mouse IgM (u chain specific)
• Specificity: No reaction was observed against anti-Mouse IgG F(c). Some light chain cross reactivity will occur with anti-Mouse IgG.
• Isotype: IgM
• Host: Mouse
• Gene: IGHM
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: Lyophilized from 0.06 M Potassium Phosphate, 0.45 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 500 ul deionized water (or equivalent).

Alternate Names for Mouse IgM Isotype Control [FITC]

• AGM1
• DKFZp686I15196
• DKFZp686I15212
• FLJ00385
• IgM
• immunoglobulin heavy constant mu
• MGC104996
• MGC52291
• MU
• VH

Background

IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to its large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to its high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGHM