Mouse IgM Isotype Control

SDS-Page: Mouse IgM Isotype Control [NBP1-97007] - Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule. Lane 3: Reduced Mouse F(c) Fragment. Lane 4: Reduced Mouse F(ab) Fragment. Lane 5: Mouse IgM Whole Molecule. Load: 1 ug per lane.

SDS-Page: Mouse IgM Isotype Control [NBP1-97007] - Lane 1: Mouse IgM, Non-Reduced. Lane 2: Mouse IgM, Reduced. Load: 1.0 ug per lane.

• Reactivity: Mu
• Applications: WB, ELISA, PAGE
• Host: Mouse
• Conjugate: Unconjugated
• Format: Purified

Mouse IgM Isotype Control Summary

• Description: This product was prepared from normal serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum and anti-Mouse IgM (u chain specific)
  
  Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Specificity: No reaction was observed against anti-Mouse IgG F(c). Some light chain cross-reactivity will occur with anti-Mouse IgG.
• Isotype: IgM
• Host: Mouse
• Gene: IGHM
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • SDS-Page 1:100 - 1:2000
  • Western Blot 1:100 - 1:2000
• Application Notes: This product has been tested by SDS-Page and can be utilized as a control or standard reagent in SDS, Western Blotting, and ELISA experiments.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
• Preservative: 0.1% Sodium Azide
• Purity: Multi-step

Alternate Names for Mouse IgM Isotype Control

• AGM1
• DKFZp686I15196
• DKFZp686I15212
• FLJ00385
• IgM
• immunoglobulin heavy constant mu
• MGC104996
• MGC52291
• MU
• VH

Background

Mouse IgM, or mouse Immunoglobulin M, purified protein is a basic antibody that is produced by B cells. Mouse IgM is the primary antibody against A and B antigens on red blood cells. Mouse IgM is by far the physically largest antibody in the human circulatory system. Mouse IgM is the first antibody to appear in response to initial exposure to antigen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGHM