IgM Heavy Chain Antibody (IM260 + IM373 + ICO-30)

Immunohistochemistry-Paraffin: IgM Heavy Chain Antibody (IM260 + IM373 + ICO-30) [NBP2-47255] - Formalin-fixed, paraffin-embedded human Tonsil stained with IgM Monoclonal Antibody (IM260 + IM373 + ICO-30)

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IHC-P
• Clone: IM260 + IM373 + ICO-30
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

IgM Heavy Chain Antibody (IM260 + IM373 + ICO-30) Summary

• Immunogen: Recombinant heavy chain of human IgM (IM260 & IM373); Heavy chain of human IgM (ICO-30)
• Localization: Cytoplasm, Cell Surface and Secreted
• Marker: B-Cell Marker
• Specificity: Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
• Isotype: IgG1/IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGHM
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 - 1 ug/ml
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1ml
  • Immunocytochemistry/Immunofluorescence 0.5 - 1 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1 ug/ml
• Application Notes: Human Chromosome Location: 14q32.33
  Mol. Weight of Antigen: 50-75 kDa
  Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for IgM Heavy Chain Antibody (IM260 + IM373 + ICO-30)

• Constant region of heavy chain of IgM
• Ig Mu Chain C Region BOT
• Ig Mu Chain C Region GAL
• Ig Mu Chain C Region OU
• immunoglobulin heavy constant mu
• Immunoglobulin mu chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGHM