Rabbit anti-Porcine IgM Heavy Chain Secondary Antibody

Product Discontinued

Rabbit anti-Porcine IgM Heavy Chain Secondary Antibody Summary

• Description: This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
• Immunogen: Swine IgM mu heavy chain
• Specificity: No reaction was observed against Swine IgG.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IGHM
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000 - 1:100000
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000 - 1:10000
• Application Notes: This secondary antibody are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Notes

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Swine IgM and Swine Serum

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Rabbit anti-Porcine IgM Heavy Chain Secondary Antibody

• Constant region of heavy chain of IgM
• Ig Mu Chain C Region BOT
• Ig Mu Chain C Region GAL
• Ig Mu Chain C Region OU
• immunoglobulin heavy constant mu
• Immunoglobulin mu chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgM Antibody (NBP1-73956)(1)

Publication using NBP1-73956

• Sadeghipour H, Torabi R, Gottschall J et al. Blockade of IgM-Mediated Inflammation Alters Wound Progression in a Swine Model of Partial-Thickness Burn J Burn Care Res 2016-11-02 [PMID: 27801682] (IHC-P)

Bioinformatics

• Gene Symbol: IGHM