Rabbit anti-Porcine IgM Secondary Antibody [HRP]

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Product Details

Summary
Reactivity PoSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP
Concentration
LYOPH

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Catalog# & Conjugate Size Price

Rabbit anti-Porcine IgM Secondary Antibody [HRP] Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by cross adsorption and extensive dialysis against the buffer stated above.
Immunogen
Swine IgM u mu chain
Specificity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by cross adsorption and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Swine IgM and Swine Serum. No reaction was observed against Swine IgG.
Clonality
Polyclonal
Host
Rabbit
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:10000
  • ELISA 1:10000 - 1:50000
  • Immunohistochemistry 1:500 - 1:2500
Application Notes
This product has been assayed against 1.0 ug of Swine IgM in a standard capture ELISA using ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:2,000 - 1:8,000 of the reconstitution concentration is suggested for this product.
Readout System

Reactivity Notes

Reacts with Porcine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Label Molecular Weight: 40,000, Restore with 2.0 mL of deionized water (or equivalent).

Alternate Names for Rabbit anti-Porcine IgM Secondary Antibody [HRP]

  • AGM1
  • DKFZp686I15196
  • DKFZp686I15212
  • FLJ00385
  • IgM
  • immunoglobulin heavy constant mu
  • MGC104996
  • MGC52291
  • MU
  • VH

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for Porcine IgM Antibody (NBP1-73908). (Showing 1 - 1 of 1 FAQ).

  1. Our customer would like to know if NB718 would react to other porcine serum proteins, such as IgG. If there is any further information about its specificity, please share with us. How about other porcine serum proteins, such as IgG? The customer would like to know if NB718 would cross react with IgM, IgA and he also would like to know if it is made from the full-length porcine IgM, or just part of IgM. The customer still has few more questions: 1. The purification of NB718 is described as Immunogen affinity purified on the product page. The customer would like to know further about how the purification is proceeded.2. Based on its datasheet, this antibody can be used for IHC and ELISA. May we have the QC results of NB718 for the customer's reference? 3. Concerning specificity, what the difference is between NB718 and NBP1-73908?
    • To answer your question, NB718 would react specifically with pig IgM but it may cross react with IgM from other species as well. So NB718 is specific to IgM and would not react with IgG and it was made of heavy chain IgM. With regards to the question No.1, NB718 was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP) but in order to provide you with more detailed information on purification process and QC data, I have to contact our lab and I will be in touch with you as soon as I have the answer. NBP1-73908 is an anti-SWINE IgM (mu) antibody that has been generated in rabbit. As far as the specificity is concerned, both antibodies are the same except the host they were generated in.

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Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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