Rabbit anti-Human IgM Heavy Chain Secondary Antibody [HRP]

Product Discontinued

Rabbit anti-Human IgM Heavy Chain Secondary Antibody [HRP] Summary

• Immunogen: Human IgM mu heavy chain
• Specificity: No reaction was observed against Human IgG.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IGHM
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000 - 1:50000
  • Immunohistochemistry 1:500 - 1:2500
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000 - 1:10000
• Application Notes: This product has been assayed against 1.0 ug of Human IgM in a standard capture ELISA using ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:1,000 - 1:3,000 of the reconstitution concentration is suggested for this product.
  
  NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store at -20C in the dark. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Gentamicin Sulfate
• Purity: Ion exchange chromatography

Notes

Label Molecular Weight: 40,000, Restore with 2.0 mL of deionized water (or equivalent).

Alternate Names for Rabbit anti-Human IgM Heavy Chain Secondary Antibody [HRP]

• Constant region of heavy chain of IgM
• Ig Mu Chain C Region BOT
• Ig Mu Chain C Region GAL
• Ig Mu Chain C Region OU
• immunoglobulin heavy constant mu
• Immunoglobulin mu chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGHM