Rabbit anti-Feline IgM Secondary Antibody [FITC]

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Flow Cytometry: Rabbit anti-Feline IgM Secondary Antibody [Fluorescein] [NBP1-73836] - Phenotyping of cat blood. Cat blood cells were washed and incubated with the antibody. It was used as a secondary antibody to check ...read more

Product Details

Summary
Reactivity FeSpecies Glossary
Applications FLISA, Flow, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
FITC
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price

Rabbit anti-Feline IgM Secondary Antibody [FITC] Summary

Description
This antibody is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Immunogen
Cat IgM mu heavy chain
Specificity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Cat IgM and Cat Serum. No reaction was observed against Cat IgG.
Clonality
Polyclonal
Host
Rabbit
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:1:10 - 1:1000
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000
Reviewed Applications
Read 1 Review rated 3
using
NBP1-73836 in the following applications:

Reactivity Notes

Reacts with Feline.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Emission Wavelength: 528, Excitation Wavelength: 495, Label Molecular Weight: 390, Restore with 2.0 mL of deionized water (or equivalent).

Alternate Names for Rabbit anti-Feline IgM Secondary Antibody [FITC]

  • AGM1
  • DKFZp686I15196
  • DKFZp686I15212
  • FLJ00385
  • IgM
  • immunoglobulin heavy constant mu
  • MGC104996
  • MGC52291
  • MU
  • VH

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgM Antibody (NBP1-73836) (0)

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Review for IgM Antibody (NBP1-73836) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Feline.

Reviews using NBP1-73836:
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Flow
(1)
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Feline
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry IgM NBP1-73836
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reviewed by:
Anonymous
Flow Feline 02/01/2018
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Summary

ApplicationFlow Cytometry
Sample TestedWhole blood cells
SpeciesFeline
Lot4131

Comments

CommentsCat blood cells were washed and incubated with the antibody. It was used as a secondary antibody to check for bound IgM, in cat blood. It served as a B cell marker.

Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for IgM Antibody (NBP1-73836) (0)

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Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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Recent Reviews

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Anonymous
02/01/2018
Application: Flow
Species: Feline