Goat anti-Monkey IgM Secondary Antibody [HRP]

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Western Blot: Goat anti-Monkey IgM Secondary Antibody [HRP] - [NBP1-73726] - Lane 1: Monkey IgM. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at ...read more

Product Details

Summary
Reactivity MkSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
LYOPH

Goat anti-Monkey IgM Secondary Antibody [HRP] Summary

Immunogen
Monkey IgM m chain
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Monkey IgM and Monkey Serum. No reaction was observed against Monkey IgA or Monkey IgG.
Isotype
IgM
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • ELISA 1:50000 - 1:100000
  • Immunohistochemistry 1:500-1:2500
  • Immunohistochemistry-Paraffin 1:500-1:2500
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free.
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 1.0 ml deionized water.

Notes

Restore with 1.0 mL of deionized water (or equivalent).

Alternate Names for Goat anti-Monkey IgM Secondary Antibody [HRP]

  • AGM1
  • DKFZp686I15196
  • DKFZp686I15212
  • FLJ00385
  • IgM
  • immunoglobulin heavy constant mu
  • MGC104996
  • MGC52291
  • MU
  • VH

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for IgM Antibody (NBP1-73726). (Showing 1 - 2 of 2 FAQ).

  1. Can this antibody be used with samples from the Rhesus monkey or cross re-activity with Rhesus monkey ?
    • This product is generally specific for Monkey IgM's which includes mostly Old World monkeys, like: Macaque, Cynomologus, Rhesus, Baboon, Chimpanzee, Great Ape and any other African species. Reactivity to New World primates has not yet been determined.
  2. Will this antibody cross react with Human IgM?
    • Although it has not been specifically tested for human IgM, this antibody is predicted to cross react with human IgM. 

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Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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