Goat anti-Monkey IgM Heavy Chain Secondary Antibody [Alkaline Phosphatase]

Western Blot: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [Alkaline Phosphatase] [NBP1-72664] - Lane 1: Monkey IgM. Lane 2: None. Load: 100 ng per lane. Primary antibody: None. Secondary antibody: Alkaline Phosphatase goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 72 kDa, 72 kDa for Monkey IgM. Other band(s): Monkey IgM splice variants and isoforms.

Western Blot: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [Alkaline Phosphatase] [NBP1-72664] - This table displays additional reactivity among various species of serum and immunoglobulin. A (+) indicates antibody reactivity to the corresponding target.

• Reactivity: Pm
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alkaline Phosphatase

Goat anti-Monkey IgM Heavy Chain Secondary Antibody [Alkaline Phosphatase] Summary

• Description: Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
  
  This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Monkey IgM and Monkey Serum
• Immunogen: Monkey IgM whole molecule
• Specificity: No reaction was observed against other Monkey heavy or light chain proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGHM
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000 - 1:10000
  • Immunohistochemistry 1:200 - 1:1000
  • Western Blot 1:500 - 1:3000
• Application Notes: This product has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Specific conditions for reactivity should be optimized by the end user.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Alternate Names for Goat anti-Monkey IgM Heavy Chain Secondary Antibody [Alkaline Phosphatase]

• Constant region of heavy chain of IgM
• Ig Mu Chain C Region BOT
• Ig Mu Chain C Region GAL
• Ig Mu Chain C Region OU
• immunoglobulin heavy constant mu
• Immunoglobulin mu chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGHM