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Rabbit anti-Rat IgM Secondary Antibody [DyLight 550]

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 550

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alkaline Phosphatase
NBP1-72689
Allophycocyanin/Cy7
NBP1-72790APCCY7-0.5ml
Biotin
NBP1-73709
DyLight 405
NBP1-72790V
DyLight 488
NBP1-72790G
DyLight 594
NBP1-72790DL594
DyLight 650
NBP1-72790C
DyLight 680
NBP1-72790FR
DyLight 755
NBP1-72790IR
FITC
NBP1-73895-10mg
FITC
NBP1-73380
HRP
NBP1-73897-10mg
HRP
NBP1-73724
Janelia Fluor 549
NBP1-72790JF549
Janelia Fluor 646
NBP1-72790JF646
PE/Atto594
NBP1-72790PEATT594-0.5ml
PE/Cy5.5
NBP1-72790PECY55-0.5ml
PE/Cy7
NBP1-72790PECY7-0.5ml
Rhodamine
NBP1-73379
Texas Red
NBP1-73710
Unconjugated
NBP1-72790
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Rabbit anti-Rat IgM Secondary Antibody [DyLight 550] Summary

Immunogen
Rat IgM whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Rat IgM and Rat Serum. No reaction was observed against other rat heavy or light chain proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IGHM
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot : 1:5000 - 1:25000
  • ELISA : 1:50000 - 1:250000
  • Immunohistochemistry : 1:1000 - 1:5000
  • Immunohistochemistry-Frozen : 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin : 1:1000 - 1:5000
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Rabbit anti-Rat IgM Secondary Antibody [DyLight 550]

  • AGM1
  • DKFZp686I15196
  • DKFZp686I15212
  • FLJ00385
  • IgM
  • immunoglobulin heavy constant mu
  • MGC104996
  • MGC52291
  • MU
  • VH

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: MLC1 Antibody [NBP1-81555] - Analysis in human cerebral cortex and pancreas tissues using NBP1-81555 antibody. Corresponding MLC1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: MLC1 Antibody [NBP1-81555] - Staining of human cerebellum shows moderate cytoplasmic positivity in neuropil of the molecular layer.
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RGM-C/Hemojuvelin
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RGM‑C/Hemojuvelin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human/Mouse RGM‑C/Hemojuvelin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3720) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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RUNX1T1/ETO
Antibody
NBP2-55747
Western Blot: RUNX1T1/ETO Antibody [NBP2-55747] - H2135 and H2087 non-small cell lung cancer cells showing baseline Runx1T1 expression versus Runx1T1 stable overexpression. Image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: RUNX1T1/ETO Antibody [NBP2-55747] - Staining of human cell line HEK 293 shows localization to nucleoplasm. Antibody staining is shown in green.
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Aldo-keto
Reductase 1C4/AKR1C4 ...
H00001109-M01
Western Blot: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Aldo-keto Reductase 1C4/AKR1C4 monoclonal antibody (M01), clone 2C11 Western Blot analysis of Aldo-keto Reductase 1C4/AKR1C4 expression in MCF-7 [L046V1].
Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Analysis of monoclonal antibody to AKR1C4 on HepG2 cell. Antibody concentration 15 ug/ml.
Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Analysis of monoclonal antibody to AKR1C4 on formalin-fixed paraffin-embedded human liver tissue. Antibody concentration 3 ug/ml.
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beta-Galactosidase-1/G ...
NBP2-45731
Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GLB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: beta-Galactosidase-1/GLB1 Antibody (1C9) [NBP2-45731] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: beta-Galactosidase-1/GLB1 Antibody (OTI1C9) [NBP2-45731] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLB1.
(5 Publications)
Species: All-NA
Applications: WB, IHC, IHC-P
IFN-alpha 1
Antibody
NBP2-75930
Western Blot: IFN-alpha 1 Antibody [NBP2-75930] - analysis of IFNA1 on mouse liver tissue lysate using anti-IFNA1 antibody at 1/100 dilution.
Immunocytochemistry/Immunofluorescence: IFN-alpha 1 Antibody [NBP2-75930] - staining IFNA1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: IFN-alpha 1 Antibody [NBP2-75930] - analysis of paraffin-embedded mouse brain tissue tissue using anti-IFNA1 antibody. Counter stained with hematoxylin.
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CDR2 Antibody (33)
NBP2-10509
Western Blot: CDR2 Antibody (33) [NBP2-10509] - Western blot analysis of CDR2 expression in 1) HeLa control cell lysate and 2) HeLa cell lysate overexpressing CDR2.
Immunohistochemistry: CDR2 Antibody (33) [NBP2-10509] - IHC staining of CDR2 in human breast cancer using DAB with hematoxylin counterstain.
Western Blot: CDR2 Antibody (33) [NBP2-10509] - Western blot analysis of CDR2 expression in 1) HeLa, 2) NTERA-2 and 3) HepG2 whole cell lysates.
(1 Publication)
Species: Hu, Mu(-)
Applications: WB, ELISA, IHC, IHC-P, IP
CD5 Antibody
[Unconjugated]
AF1636
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Simple Western lane view shows lysates of CEM human T-lymphoblastoid cell line, loaded at 0.2 mg/mL. A specific band was detected for CD5 at approximately 93 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CD5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1636). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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CTLA-4
[Unconjugated]
7268-CT
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(1 Publication)
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Glutathione S
Transferase kappa 1 A ...
NBP2-80461
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SLCO6A1 Antibody
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MKP-1/DUSP1
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NBP2-67909
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HLA DQ Antibody
(SPV-L3)
NBP2-45041
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NOD2 Antibody (2D9)
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.
Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
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Complement C4b/d
Antibody (16D2)
NB200-541
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ARC/NOL3 Antibody
NBP2-19561
Western Blot: ARC/NOL3 Antibody [NBP2-19561] - Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE diluted at 1:1000
Immunocytochemistry/Immunofluorescence: ARC/NOL3 Antibody [NBP2-19561] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using NOL3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Immunohistochemistry-Frozen: ARC/NOL3 Antibody [NBP2-19561] - NOL3 antibody detects NOL3 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse cerebellum. Green: NOL3 stained by NOL3 antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
Species: Hu, Mu, Rt
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ARC/ARG3.1 Antibody
NBP1-56929
Western Blot: ARC/ARG3.1 Antibody [NBP1-56929] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:312500 Positive Control: Transfected 293T
Immunohistochemistry-Paraffin: ARC/ARG3.1 Antibody [NBP1-56929] - Human adrenal tissue at an antibody concentration of 5ug/ml.
Western Blot: ARC/ARG3.1 Antibody [NBP1-56929] - Analysis of 293T cell lysate. Antibody Dilution: 1.0 ug/ml.
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Applications: WB, IHC, IHC-P
Rabbit anti-Rat
IgM Secondary Antibod ...
NBP1-72790R
Rabbit anti-Rat IgM Secondary Antibody [DyLight 550]
Species: Rt
Applications: WB, ELISA, IHC, IHC-Fr, IHC-P

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Product General Protocols

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Other Available Formats

Alkaline Phosphatase NBP1-72689
Allophycocyanin/Cy7 NBP1-72790APCCY7
Biotin NBP1-73709
DyLight 405 NBP1-72790V
DyLight 488 NBP1-72790G
DyLight 550 NBP1-72790R
DyLight 594 NBP1-72790DL594
DyLight 650 NBP1-72790C
DyLight 680 NBP1-72790FR
DyLight 755 NBP1-72790IR
FITC NBP1-73895
FITC NBP1-73380
HRP NBP1-73897
HRP NBP1-73724
Janelia Fluor 549 NBP1-72790JF549
Janelia Fluor 646 NBP1-72790JF646
PE/Atto594 NBP1-72790PEATT594
PE/Cy5.5 NBP1-72790PECY55
PE/Cy7 NBP1-72790PECY7
Rhodamine NBP1-73379
Texas Red NBP1-73710
Learn about Custom Antibody Labeling

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Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY licence.
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Bioinformatics

Gene Symbol IGHM