Rabbit anti-Rat IgM Heavy Chain Secondary Antibody

Western Blot: Rabbit anti-Rat IgM Heavy Chain Secondary Antibody [NBP1-72790] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (RABBIT) Antibody 1:2,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.

Western Blot of Rabbit anti-Rat IgM Heavy Chain Secondary Antibody

• Reactivity: Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Rabbit anti-Rat IgM Heavy Chain Secondary Antibody Summary

• Description: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose followed by solid phase adsorption(s). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Rat IgM and Rat Serum
  
  Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Immunogen: Rat IgM whole molecule
• Specificity: No reaction was observed against other rat heavy or light chain proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IGHM
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:50000 - 1:250000
  • Immunohistochemistry 1:1000 - 1:5000
  • Western Blot 1:5000 - 1:25000
• Application Notes: This product is tested by western blot and suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Alternate Names for Rabbit anti-Rat IgM Heavy Chain Secondary Antibody

• Constant region of heavy chain of IgM
• Ig Mu Chain C Region BOT
• Ig Mu Chain C Region GAL
• Ig Mu Chain C Region OU
• immunoglobulin heavy constant mu
• Immunoglobulin mu chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGHM