Goat anti-Ferret IgM Heavy Chain Secondary Antibody [Alkaline Phosphatase] Summary
| Immunogen |
Ferret IgM whole molecule |
| Specificity |
No reaction was observed against other ferret heavy or light chain proteins. |
| Isotype |
IgG |
| Clonality |
Polyclonal |
| Host |
Goat |
| Gene |
IGHM |
| Purity |
Multi-step |
Applications/Dilutions
| Dilutions |
- ELISA 1:1000 - 1:2500
- Immunohistochemistry 1:200 - 1:1000
- Western Blot 1:500 - 1:2500
|
| Application Notes |
This secondary antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. |
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze. |
| Buffer |
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative |
0.01% Sodium Azide |
| Purity |
Multi-step |
Notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Ferret IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Ferret IgM and Ferret Serum
Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Alternate Names for Goat anti-Ferret IgM Heavy Chain Secondary Antibody [Alkaline Phosphatase]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are
guaranteed for 1 year from date of receipt.
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