Home » IgM » IgM Antibodies » Goat anti-Feline IgM Secondary Antibody

Goat anti-Feline IgM Secondary Antibody

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Flow Cytometry: Goat anti-Feline IgM Secondary Antibody [NB7258] - The cat cells were washed and Incubated with the IgM antibody. It was used to detect Bcells. This image was submitted via customer review. Image from the FITC version of this antibody.
Flow Cytometry: Goat anti-Feline IgM Secondary Antibody [NB7258] - The cat cells were washed and Incubated with the IgM antibody. It was used to detect Bcells. This image was submitted via customer review. Image from ...read more

Product Details

Summary
Reactivity FeSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
FITC
NB7259
HRP
NB7260
Janelia Fluor 549
NB7258JF549
Janelia Fluor 646
NB7258JF646
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Goat anti-Feline IgM Secondary Antibody Summary

Immunogen
Cat IgM
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with cat IgM. This may cross react with IgM from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000- 1:30000
  • ELISA 1:1000 - 1:30000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:2000
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
Application Notes
ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500
Publications
Read Publications using NB7258.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Goat anti-Feline IgM Secondary Antibody

  • AGM1
  • DKFZp686I15196
  • DKFZp686I15212
  • FLJ00385
  • IgM
  • immunoglobulin heavy constant mu
  • MGC104996
  • MGC52291
  • MU
  • VH

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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CTLA‑4 was detected in immersion fixed human peripheral blood mononuclear cells treated with treated with PMA and calcium ionomycin using Goat Anti-Human CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Goat anti-Feline
IgM Secondary Antibod
NB7258
Goat anti-Feline IgM Secondary Antibody
Species: Fe
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P

Publications for IgM Antibody (NB7258)(5)


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Product General Protocols

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WB Video Protocol
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Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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