Goat anti-Feline IgM Secondary Antibody

Flow Cytometry: Goat anti-Feline IgM Secondary Antibody [NB7258] - The cat cells were washed and Incubated with the IgM antibody. It was used to detect Bcells. This image was submitted via customer review. Image from the FITC version of this antibody.

• Reactivity: Fe
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Goat anti-Feline IgM Secondary Antibody Summary

• Immunogen: Cat IgM
• Specificity: By immunoelectrophoresis and ELISA this reacts specifically with cat IgM. This may cross react with IgM from other species.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000- 1:30000
  • ELISA 1:1000 - 1:30000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:2000
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
• Application Notes: ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Goat anti-Feline IgM Secondary Antibody

• AGM1
• DKFZp686I15196
• DKFZp686I15212
• FLJ00385
• IgM
• immunoglobulin heavy constant mu
• MGC104996
• MGC52291
• MU
• VH

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgM Antibody (NB7258)(5)

Publications using NB7258

• Zulpo DL, Headley SA, Biazzono L et al. Oocyst shedding in cats vaccinated by the nasal and rectal routes with crude rhoptry proteins of Toxoplasma gondii. Exp Parasitol. [PMID: 22542988]
  
  Details:
  Citation using the HRP form of this antibody.
• Romen F, Pawlita M, Sehr P et al. Antibodies against Gag are diagnostic markers for feline foamy virus infections while Env and Bet reactivity is undetectable in a substantial fraction of infected cats. Virology. [PMID: 16297422]
  
  Details:
  Citation using the HRP form of this antibody.
• Burkhard MJ, Hoover EA. IgG from acutely infected cats blocks mucosal feline immunodeficiency virus infection. Vet Immunol Immunopathol. [PMID: 15910995]
  
  Details:
  Citation using the HRP form of this antibody.
• Fujino Y, Ma Z, Satoh H et al. Characterization of a newly established nonproducer lymphoma cell line for feline leukemia virus. Vet Immunol Immunopathol 2004 Dec [PMID: 15541796]
• Omori M, Pu R, Tanabe T et al. Cellular immune responses to feline immunodeficiency virus (FIV) induced by dual-subtype FIV vaccine. Vaccine 2004 Dec [PMID: 15530685]