Human IgM Isotype Control [Alexa Fluor® 546]

• Reactivity: Hu
• Applications: Flow, ICC/IF
• Clonality: Polyclonal
• Host: Human
• Conjugate: Alexa Fluor 546

Human IgM Isotype Control [Alexa Fluor® 546] Summary

• Description: This Human IgM immunoglobulin is useful as an isotype control for Human IgM antibody.
• Isotype: IgM
• Clonality: Polyclonal
• Host: Human
• Gene: IGHM
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface) 1:10 - 1:1000
  • Flow (Intracellular) 1:10 - 1:1000
  • Flow Cytometry 1:10-1:1000
  • Immunofluorescence 1:10 - 1:500
• Application Notes: Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at -20C in the dark. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% glycerol.
• Preservative: No Preservative
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Alternate Names for Human IgM Isotype Control [Alexa Fluor® 546]

• AGM1
• DKFZp686I15196
• DKFZp686I15212
• FLJ00385
• IgM
• immunoglobulin heavy constant mu
• MGC104996
• MGC52291
• MU
• VH

Background

IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to its large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to its high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGHM