IgM Antibody (SPM557) [DyLight 550]

Images

 
Immunocytochemistry/ Immunofluorescence: IgM Antibody (SPM557) [DyLight 550] [NBP2-34422R] - ICC analysis of LM7 cells using anti-IgM antibody [DyLight 550]. DAPI Solution (blue; cat.# NBP2-31156) was used as a nuclear ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P
Clone
SPM557
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 550

IgM Antibody (SPM557) [DyLight 550] Summary

Immunogen
Recombinant heavy chain of human IgM
Localization
Cytoplasm, Cell Surface and Secreted
Marker
B-Cell Marker
Specificity
Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IGHM
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-34422R in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for IgM Antibody (SPM557) [DyLight 550]

  • AGM1
  • DKFZp686I15196
  • DKFZp686I15212
  • FLJ00385
  • IgM
  • immunoglobulin heavy constant mu
  • MGC104996
  • MGC52291
  • MU
  • VH

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgM Antibody (NBP2-34422R) (0)

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Review for IgM Antibody (NBP2-34422R) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ICC.

Reviews using NBP2-34422R:
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(1)
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(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry IgM NBP2-34422R
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reviewed by:
Arun Satelli
ICC Human 10/07/2014
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman Cancer cell line LM7
SpeciesHuman
CommentsThis antibody was tested against IgM antibody. Gave 4 stars since I didnt stain at 1:1000.

Blocking

Blocking Details0.25% FBS in PBS

Primary Anitbody

Dilution Ratio1:500

Details

Detection NotesDetection using confocal microscope LSM510
Fixation DetailsFixing using 4% PFA, Block in 0.25% FBS,
Wash Description5 min each 3 times

Comments

CommentsThis antibody was tested against IgM antibody. Gave 4 stars since I didnt stain at 1:1000.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgM Antibody (NBP2-34422R) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for IgM Antibody (NBP2-34422R)

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Diseases for IgM Antibody (NBP2-34422R)

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Blogs on IgM.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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Recent Reviews

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4
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1
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Arun Satelli
10/07/2014
Application: ICC
Species: Human

Bioinformatics

Gene Symbol IGHM