IgM Antibody (ICO-30) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, IHC-P, ICC/IF
Clone
ICO-30
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

IgM Antibody (ICO-30) [Janelia Fluor® 549] Summary

Immunogen
Heavy chain of human IgM
Localization
Cytoplasm, Cell Surface and Secreted
Marker
B-Cell Marker
Specificity
Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. IgM antibody is prominent in early immune responses to most antigens. Aberrant levels are associated with immune deficiency states, hereditary deficiencies, myeloma, Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
IGHM
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for IgM Antibody (ICO-30) [Janelia Fluor® 549]

  • AGM1
  • DKFZp686I15196
  • DKFZp686I15212
  • FLJ00385
  • IgM
  • immunoglobulin heavy constant mu
  • MGC104996
  • MGC52291
  • MU
  • VH

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu
Applications: Flow, IHC, IHC-P, ICC/IF

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47723AF350
Alexa Fluor 405 NBP2-47723AF405
Alexa Fluor 488 NBP2-47723AF488
Alexa Fluor 532 NBP2-47723AF532
Alexa Fluor 594 NBP2-47723AF594
Alexa Fluor 647 NBP2-47723AF647
Alexa Fluor 700 NBP2-47723AF700
Alexa Fluor 750 NBP2-47723AF750
Allophycocyanin NBP2-47723APC
Allophycocyanin/Cy7 NBP2-47723APCCY7
Biotin NBP2-47723B
DyLight 350 NBP2-47723UV
DyLight 405 NBP2-47723V
DyLight 488 NBP2-47723G
DyLight 550 NBP2-47723R
DyLight 594 NBP2-47723DL594
DyLight 650 NBP2-47723C
DyLight 680 NBP2-47723FR
DyLight 755 NBP2-47723IR
FITC NBP2-47723F
HRP NBP2-47723H
Janelia Fluor 549 NBP2-47723JF549
Janelia Fluor 646 NBP2-47723JF646
PE NBP2-47723PE
PE/Atto594 NBP2-47723PEATT594
PE/Cy5.5 NBP2-47723PECY55
PE/Cy7 NBP2-47723PECY7
PerCP NBP2-47723PCP

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Bioinformatics Tool for IgM Antibody (NBP2-47723JF549)

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Diseases for IgM Antibody (NBP2-47723JF549)

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Blogs on IgM.

How to identify B cell subsets using flow cytometry
By Victoria OsinskiUsing flow cytometry to identify B cell subsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified a...  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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Bioinformatics

Gene Symbol IGHM
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