Mouse IgG3 Isotype Control (MG3) [FITC]

Dot Blot: Mouse IgG3 Isotype Control (MG3) [FITC] [NBP1-96847] - Mouse IgG3 Protein [Fluorescein] [NBP1-96847] - A three-fold serial dilution of Fluorescein conjugated Mouse IgG3 subclass starting at 200ug was spotted onto 0.45 um nitrocellulose.

Dot Blot showing the detection Fluorescein conjugated Mouse IgG3 subclass. A three-fold serial dilution of Mouse IgG3 Isotype Control (MG3) [FITC]starting at 200ng was spotted onto 0.45 ?m nitrocellulose and imaged using the Bio-Rad VersaDoc(R) 4000 MP.

• Reactivity: Mu
• Applications: WB, DB, ELISA, Flow, ICC/IF, IHC
• Clone: MG3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC
• Concentration: LYOPH

Mouse IgG3 Isotype Control (MG3) [FITC] Summary

• Description: Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  This product has been prepared from serum-free tissue culture supernate by protein A chromatography
• Specificity: Typically less than 1% cross reactivity against other mouse heavy or light chain specific antibodies was detected by ELISA.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGHG3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:1000 - 1:5000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Western Blot 1:100 - 1:2000
• Application Notes: This product has been tested in dot blot and is designed for immunofluorescence microscopy, flow cytometry, fluorescence based plate assays (FLISA), fluorescent western blotting, multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent).

Alternate Names for Mouse IgG3 Isotype Control (MG3) [FITC]

• DKFZp686H11213
• FLJ39988
• FLJ40036
• FLJ40253
• FLJ40587
• FLJ40789
• FLJ40834
• IgG3
• immunoglobulin heavy constant gamma 3 (G3m marker)
• MGC45809

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGHG3