Mouse IgG3 Isotype Control (M3A) [Texas Red]

Product Discontinued

Mouse IgG3 Isotype Control (M3A) [Texas Red] Summary

• Description: This Mouse IgG3 immunoglobulin is useful as an isotype control for Mouse IgG3 antibody.
• Specificity: In an Ouchterlony double diffusion assay the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgM , and IgA
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGHG3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000 - 1:5000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:1000 - 1:5000
• Application Notes: This isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent).

Notes

This product has been prepared from mouse ascitic fluid by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum.

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Mouse IgG3 Isotype Control (M3A) [Texas Red]

• DKFZp686H11213
• FLJ39988
• FLJ40036
• FLJ40253
• FLJ40587
• FLJ40789
• FLJ40834
• IgG3
• immunoglobulin heavy constant gamma 3 (G3m marker)
• MGC45809

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGHG3