IGFLR1/TMEM149 Antibody (905338) [Unconjugated]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human IGFLR1 Monoclonal Antibody (Catalog # MAB8296) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

• Reactivity: Hu
• Applications: Flow, B/N, CyTOF-ready
• Clone: 905338
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IGFLR1/TMEM149 Antibody (905338) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived recombinant human IGFLR1
  Met1-Pro163
  Accession # Q9H665
• Specificity: Detects human IGFLR1in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGFLR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction 0.05-0.3 ug/mL
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGFLR1/TMEM149 Antibody (905338) [Unconjugated]

• FLJ22573
• IGFLR1
• TMEM149
• transmembrane protein 149
• U2 small nuclear RNA auxiliary factor 1-like 4
• U2(RNU2) small nuclear RNA auxiliary factor 1-like 4
• U2AF1L4

Background

Insulin Growth Factor-like Family Receptor 1 (IGFLR1) is a 355 amino acid (aa) type 1a transmembrane protein that was identified in a screen for binding partners of human IGFL-1 (1). Mature human IGFLR1 consists of an extracellular domain (ECD) with two putative cysteine-rich domains (CRDs), a transmembrane region, and a cytoplasmic domain (1). Its structure has similarities to TNF receptor family members (1). Over the first 163 aa, human IGFLR1 shares 61% and 59% aa sequence identity with mouse and rat IGFLR1, respectively. In mice, IGFLR1 is expressed primarily on T cells and, similar to the human proteins, mouse IGFLR1 binds the mouse IGFL protein (1). Human IGFL-1 expression is enhanced by TNF-alpha treatment and was shown to be up-regulated in human psoriatic skin samples, suggesting that IGFLR1 may have a role during skin inflammation (1).

1. Lobito, A.A. et al. (2011) J. Biol. Chem. 286:18969.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGFLR1
• Uniprot:
  • Q9H665()