Human IgA ELISA Kit (Colorimetric) Summary
| Description |
Assay Length: 1-5 hours |
| Specificity |
This assay has high sensitivity and excellent specificity for detection of human IgA. No significant cross-reactivity or interference between human IgA and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human IgA and all the analogues, therefore, cross reaction may still exist. |
| Standard Curve Range |
0.24 ng/mL - 1000 ng/mL |
| Sensitivity |
0.24 ng/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
%CV < 8 |
| Intra-Assay |
%CV < 8 |
| Sample Volume |
50 - 100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
IGHA1 |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Adhesive Strip (For 96 wells)
- Assay plate (12 x 8 coated Microwells)
- HRP-conjugate (100 x concentrate)
- HRP-conjugate Diluent
- Instruction manual
- Sample Diluent
- Standard (Freeze dried)
- Stop Solution
- TMB Substrate
- Wash Buffer (25 x concentrate)
|
Alternate Names for Human IgA ELISA Kit (Colorimetric)
Background
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IgA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgA present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for IgA is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IgA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for IgA ELISA Kit (NBP2-60057). (Showing 1 - 1 of 1 FAQ).
-
wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.