IFN-gamma R1/CD119 Antibody (RM0093-7K23) - Azide and BSA Free

Immunohistochemistry-Paraffin: IFN-gamma R1/CD119 Antibody (RM0093-7K23) [NBP1-22408] - The Kidney tissue samples from the Folic Acid induced kidney injury model were fixed using 4% PFA at 4C for overnight and embeded in paraffin. 4um section was sublected to IHC with NBP1-22408

• Reactivity: Mu
• Applications: WB, IHC
• Clone: RM0093-7K23
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

IFN-gamma R1/CD119 Antibody (RM0093-7K23) - Azide and BSA Free Summary

• Description: Novus Biologicals Rat IFN-gamma R1/CD119 Antibody (RM0093-7K23) - Azide and BSA Free (NBP1-22408) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Mouse recombinant IFN-gamma R1/CD119
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Rat
• Gene: IFNGR1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for IFN-gamma R1/CD119 Antibody (RM0093-7K23) - Azide and BSA Free

• AVP, type 2
• CD119 antigen
• CD119
• FLJ45734
• IFN-gamma R1
• IFN-gamma receptor 1
• IFNgammaR1
• IFN-gamma-R1
• IFNGR
• IFNGR1
• IFN-gR1
• immune interferon receptor 1
• interferon gamma receptor 1
• interferon-gamma receptor alpha chain
• type 2

Background

The IFNG receptor complex is composed of two type I membrane proteins, IFNGR1 (IFNGR alpha) and IFNGR2 (IFNGR beta). Both receptor proteins are members of the type II cytokine receptor family and share 52% overall sequence identity. The IFNG receptor is constitutively expressed in most cell types. Soluble IFNG has been detected in biological fluids. IFNGR1 is required for ligand-binding and signaling. It binds to IFNG with high affinity and is a potent IFNG antagonist. In signal transduction, IFNG induces tyrosine phosphorylation of IFNGR1 leading to the formation of a docking site on the activated receptor for Stat1, which specifically activates IFNG-induced gene transcription. Human IFNGR1, a 227 amino acid residue protein, has a calculated molecular mass of approximately 25 kDa. As a result of glycosylation, the recombinant IFNGR1 migrates as a 40 to 50 kDa protein in SDS-PAGE. Human and mouse IFNGRI share approximately 52% amino acid sequence identity and each interacts with IFNG in a strictly species-specific manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IFNGR1
• Entrez:
  • 3459(Human)