IFN-gamma R1/CD119 Antibody (2B9Q1)

Western Blot: IFN-gamma R1/CD119 Antibody (2B9Q1) [NBP3-15421] - Western blot analysis of extracts of various cell lines, using IFN-gamma R1/CD119 Rabbit mAb (NBP3-15421) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry/Immunofluorescence: IFN-gamma R1/CD119 Antibody (2B9Q1) [NBP3-15421] - Immunofluorescence analysis of THP-1 cells using IFN-gamma R1/CD119 Rabbit mAb (NBP3-15421) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western Blot: IFN-gamma R1/CD119 Antibody (2B9Q1) [IFN-gamma R1/CD119] - Western blot analysis of various lysates, using [KO Validated] IFN-gamma R1/CD119 Rabbit mAb at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 180s.

Western Blot: IFN-gamma R1/CD119 Antibody (2B9Q1) [IFN-gamma R1/CD119] - Western blot analysis of lysates from wild type(WT) and IFN-gamma R1/CD119 knockout (KO) 293T cells, using [KO Validated] IFN-gamma R1/CD119 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Enhanced Kit .Exposure time: 180s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 2B9Q1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

IFN-gamma R1/CD119 Antibody (2B9Q1) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 270-489 of human IFN-gamma R1/CD119 (NP_000407.1). IKKINPLKEKSIILPKSLISVVRSATLETKPESKYVSLITSYQPFSLEKEVVCEEPLSPATVPGMHTEDNPGKVEHTEELSSITEVVTTEENIPDVVPGSHLTPIERESSSPLSSNQSEPGSIALNSYHSRNCSESDHSRNGFDTDSSCLESHSSLSDSEFPPNNKGEIKTEGQELITVIKAPTSFGYDKPHVLVDLLVDDSGKESLIGYRPTEDSKEFS
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IFNGR1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for IFN-gamma R1/CD119 Antibody (2B9Q1)

• AVP, type 2
• CD119 antigen
• CD119
• FLJ45734
• IFN-gamma R1
• IFN-gamma receptor 1
• IFNgammaR1
• IFN-gamma-R1
• IFNGR
• IFNGR1
• IFN-gR1
• immune interferon receptor 1
• interferon gamma receptor 1
• interferon-gamma receptor alpha chain
• type 2

Background

IFN gamma receptor beta is part of the receptor for interferon gamma. This class II cytokine receptor pairs with CDw119 to form the IFN gamma receptor and is an integral part of the IFN gamma signal transduction pathway. CDw119 serves as the IFN gamma binding chain and associates with the IFN gamma beta chain which is required for receptor signaling. The extracellular portion of both the IFN gamma receptor alpha and beta chains must be species matched. The IFN gamma receptor beta chain is expressed on T and B cells, NK cells, monocytes/ macrophages, and fibroblasts. Binding of IFN gamma induces receptor dimerization, internalization, Jak1 and Jak2 protein kinase activation and, ultimately, STAT1 activation. It is also likely to interact with GAF. IFN gamma initiates and regulates a variety of immune responses and is required for signal transduction. Contains 2 fibronectin type III domains. Defects in IFN gamma Receptor beta are a cause of mendelian susceptibility to mycobacterial disease (MSMD), a rare condition that confers predisposition to illness caused by several mycobacteria strains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IFNGR1